Effect of butylhydroxytoluene and related compounds on permeability of the inner mitochondrial membrane

Archives of Biochemistry and Biophysics
T I GudzS A Novgorodov

Abstract

Mitochondrial inner membrane contains a latent pore (PTP) that when opened uncouples mitochondrial energy transduction and allows rapid equilibration of low-molecular-weight solutes between the matrix and exterior. Based on sensitivity of the PTP to well-known free radical scavenger butylhydroxytoluene (BHT), it has been proposed that increased steady-state level of oxygen radicals, and subsequent radical attack of proteins and lipids, is a central event in activation of this pore (Novgorodov et al., J. Bioenerg. Biomembr. 19, 191-202, 1987; Carbonera and Azzone, Biochim. Biophys. Acta 943, 245-255, 1988). Present studies revealed that DBT, a derivative of BHT devoid of radical scavenging activity, exerts an analogous effect on the permeability of the inner membrane. Inhibition of the Ca2+-induced PTP opening is essentially complete at dose range of 50-60 nmol/mg protein with IC50 values of about 32 and 23 nmol/mg protein for DBT and BHT, respectively. Electron microscopy and osmotic experiments utilizing polyethylene glycols with different Stokes radii showed that the apparent lack of inhibition seen at high concentrations of these compounds results from cyclosporin A- and Ca2+-insensitive pore formation in the inner membrane....Continue Reading

References

Jan 6, 1977·Biochimica Et Biophysica Acta·G F AzzoneP D Antone
Jul 1, 1979·Physiological Reviews·B ChanceA Boveris
Jul 1, 1979·Archives of Biochemistry and Biophysics·R A Haworth, D R Hunter
Jul 1, 1979·Archives of Biochemistry and Biophysics·D R Hunter, R A Haworth
Nov 15, 1977·Biochemical Pharmacology·G SgaragliA Giotti
Jan 1, 1992·Free Radical Biology & Medicine·C H KennedyW A Pryor
Oct 1, 1992·Free Radical Biology & Medicine·T ParaidathathuJ P Kehrer
Dec 10, 1992·Biochimica Et Biophysica Acta·M Hermes-LimaA E Vercesi
Aug 1, 1991·Journal of Bioenergetics and Biomembranes·E LenartowiczG F Azzone
Jan 1, 1989·Acta Chemica Scandinavica·N E Saris, P Somerharju
Jan 1, 1989·Methods in Enzymology·N E Saris, A Allshire
Aug 30, 1989·Biochemical and Biophysical Research Communications·K M Broekemeier, D R Pfeiffer
Dec 30, 1986·Biochemical and Biophysical Research Communications·C H KennedyD F Church
Oct 1, 1986·The Biochemical Journal·I Al-Nasser, M Crompton
Oct 26, 1988·Biochimica Et Biophysica Acta·D CarboneraG F Azzone
Apr 1, 1988·Gastroenterology·L R SchillerJ S Fordtran
Aug 18, 1988·Biochimica Et Biophysica Acta·D Carbonera, G F Azzone
May 3, 1985·Biochimica Et Biophysica Acta·A AllshireN E Saris
Sep 1, 1970·The Biochemical Journal·A L Lehninger
Sep 1, 1982·Biochimica Et Biophysica Acta·D Nicholls, K Akerman
Nov 30, 1981·Biochemical and Biophysical Research Communications·E J Harris, M B Cooper
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K W KinnallyH Tedeschi
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·M C Sorgato, O Moran
Aug 1, 1994·The American Journal of Physiology·T E GunterC E Gavin
Jun 1, 1994·Archives of Biochemistry and Biophysics·S A NovgorodovD R Pfeiffer
Apr 1, 1993·The American Journal of Physiology·T L DawsonJ J Lemasters
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Jul 4, 2008·The Journal of Biological Chemistry·Sergei A NovgorodovLina M Obeid
Jun 26, 2007·Biochemical Pharmacology·Alexey G KruglovNils-Erik L Saris
Sep 10, 2005·Biochemical Pharmacology·Katrin StaniekLars Gille
Apr 17, 1998·Biophysical Journal·J HüserL A Blatter
Oct 24, 2002·European Journal of Biochemistry·Yasuo ShinoharaHiroshi Terada
Sep 26, 1997·The Journal of Biological Chemistry·T I GudzC L Hoppel
Feb 22, 2005·The Journal of Biological Chemistry·Sergei A NovgorodovLina M Obeid
May 22, 2001·Archives of Biochemistry and Biophysics·A Sultan, P M Sokolove
May 22, 2001·Archives of Biochemistry and Biophysics·A Sultan, P M Sokolove

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