PMID: 737227Aug 1, 1978Paper

Effect of Ca2+ ions on phospholipase D interaction with mitochondrial membrane phospholipids

Biokhimii︠a︡
M M Rkahimov, K T Almatov

Abstract

Effect of phospholipase D and Ca2+ ions on mitochondrial polyenzyme systems is studied. Phospholipase D is found to have a lytic effect on mitochondrial membrane phospholipids both in the presence and in the absence of Ca2+ ions. Low Ca2+ concentrations stimulate the phospholipase D-membrane interaction, thus increasing the efficiency of the enzyme action. The degradation of mitochondrial membrane phospholipids or their heating result in a decrease of membrane binding with phospholipase D. The presence of Ca2+ protects the membranes from such a degradation. High Ca2+ concentrations cause a "pseudo" stabilization of mitochondrial polyenzyme systems with respect to phospholipase D. In fact, a decrease in phospholipase binding with mitochondrial membranes takes place at 30 mM and higher concentrations of CaCl2, which is due to the intensive phospholipid degradation at the initial reaction step. Optimal Ca2+ concentration range for the activation of phospholipase D is 2-5 mM. It does not coincide with Ca2+ concentration values necessary for the phospholipase activation with "disorganized substrates" (30-60 mM). Combined effect of Ca2+ and phospholipase D on outer and inner NADH oxidation pathway manifests differently. The inner pat...Continue Reading

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