PMID: 20632941Jul 17, 2010Paper

Effect of Ca2+ on programmed death of guard and epidermal cells of pea leaves

Biochemistry. Biokhimii︠a︡
D B KiselevskyV D Samuilov

Abstract

The effect of Ca2+ on programmed death of guard cells (GC) and epidermal cells (EC) determined from destruction of the cell nucleus was investigated in epidermis of pea leaves. Ca2+ at concentrations of 1-100 microM increased and at a concentration of 1 mM prevented the CN(-)-induced destruction of the nucleus in GC, disrupting the permeability barrier of GC plasma membrane for propidium iodide (PI). Ca2+ at concentrations of 0.1-1 mM enhanced drastically the number of EC nuclei stained by PI in epidermis treated with chitosan, an inducer of programmed cell death. The internucleosomal DNA fragmentation caused by CN(-) was suppressed by 2 mM Ca2+ on 6 h incubation, but fragmentation was stimulated on more prolonged treatment (16 h). Presumably, the disruption of the permeability barrier of plasma membrane for PI is not a sign of necrosis in plant cells. Quinacrine and diphenylene iodonium at 50 microM concentration prevented GC death induced by CN(-) or CN(-) + 0.1 mM Ca2+ but had no influence on respiration and photosynthetic O2 evolution in pea leaf slices. The generation of reactive oxygen species determined from 2',7'-dichlorofluorescein fluorescence was promoted by Ca2+ in epidermal peels from pea leaves.

References

Jan 1, 1992·Cytometry·Z DarzynkiewiczF Traganos
Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Mar 1, 1993·Immunology Today·J J Cohen
Oct 28, 1998·Nature·M J BerridgeP Lipp
Sep 11, 1999·Plant Physiology·A K Papadakis, K A Roubelakis-Angelakis
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Ian M Moller
Jul 19, 2001·Nature·J L Dangl, J D Jones
May 9, 2002·Biochemistry. Biokhimii︠a︡·S Ya ProskuryakovA G Konoplyannikov
Mar 1, 2003·The Plant Journal : for Cell and Molecular Biology·James F DatFrank Van Breusegem
Apr 1, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Lars-Oliver KlotzHelmut Sies
Sep 18, 2004·Plant Physiology·Kendal D Hirschi
Apr 18, 2006·Biochemistry. Biokhimii︠a︡·V D SamuilovA V Nesov
Jun 9, 2006·Plant Physiology·Moshe Sagi, Robert Fluhr
Jun 17, 2006·Biochimica Et Biophysica Acta·Jerzy DuszyńskiKrzysztof Zabłocki
Jul 11, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hanae KakuNaoto Shibuya
Sep 5, 2006·Apoptosis : an International Journal on Programmed Cell Death·Dmitri V KryskoPeter Vandenabeele
Nov 14, 2006·Plant Physiology and Biochemistry : PPB·M IritiF Faoro
Jan 26, 2008·The Journal of Biological Chemistry·Yoko OgasawaraKazuyuki Kuchitsu
Apr 26, 2008·Journal of Experimental Botany·Andrea KuthanovaLukas Fischer
Nov 11, 2008·Biochemistry. Biokhimii︠a︡·V D SamuilovL A Vasil'ev
Nov 18, 2009·Biochemistry. Biokhimii︠a︡·L A Vasil'evV D Samuilov
Feb 1, 2004·The New Phytologist·Anna ZuppiniPaola Mariani

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