Effect of cadmium on active ion transport and cytotoxicity in cultured renal epithelial cells (A6)

Toxicology in Vitro : an International Journal Published in Association with BIBRA
B Faurskov, H F Bjerregaard

Abstract

A cultured epithelial cell line from toad kidney (A6) was used to study the mechanism by which cadmium (Cd) affects transepithelial resistance (TER) and active transepithelial ion transport measured as short-circuit current (SCC) in vitro. The influence of Cd on cell integrity was investigated by measuring time-dependent TER under controlled conditions and the half-maximal inhibition concentration (IC(50)) 24 hr after exposure to 1 mm CdCl(2). The data suggest that Cd deterioration of cell integrity is stronger when applied to the apical relative to the basolateral solution (IC(50) = 173.9 and 147.8 muM, respectively). Also, the data demonstrate that addition of Cd to the basolateral solution results in a prompt and transient stimulation of the active ion transport from 2.6 +/- 0.4 to 8.7 +/- 1.1 muA/cm(2). Use of the sodium channel blocker amiloride indicate that Na transport is not involved in Cd-stimulated SCC. Substitution of Cl with SO(4)(2-) in the basolateral solution and use of the Cl channel inhibitors, diphenylamine-2-carboxylase (DPC) and niflumic acid indicate strongly that Cd increases Cl secretion in A6 epithelium. Thapsigargin (TG), an intracellular Ca-ATPase blocker, inhibits Cd-stimulated active ion transport i...Continue Reading

References

Jun 1, 1992·Toxicology and Applied Pharmacology·M BöhmeW Rummel
Jan 1, 1991·Toxicology and Applied Pharmacology·W C Prozialeck, R J Niewenhuis
Feb 1, 1990·Pflügers Archiv : European journal of physiology·M J StuttsR C Boucher
Jun 1, 1990·The American Journal of Physiology·H RasmussenJ S Douglas
Sep 1, 1990·Toxicology Letters·I M BruggemanJ H Temmink
Jul 1, 1989·Trends in Pharmacological Sciences·S OrreniusP Nicotera
Oct 20, 1986·Life Sciences·C V Nolan, Z A Shaikh
Sep 1, 1981·The American Journal of Physiology·F M Perkins, J S Handler
Mar 1, 1984·Environmental Health Perspectives·G F Nordberg
Sep 1, 1983·The American Journal of Physiology·S Sariban-SohrabyR J Turner
Mar 1, 1993·Experimental Physiology·N L Simmons
Jul 1, 1994·The Journal of Membrane Biology·G Inesi, Y Sagara
Aug 2, 1994·The Science of the Total Environment·L AlessioO Vesterberg
Jan 1, 1994·Pflügers Archiv : European journal of physiology·B BrodinR Nielsen
May 1, 1994·The American Journal of Physiology·B JovovN K Wills
Jan 1, 1993·Archives of Toxicology·W C Prozialeck, P C Lamar
Jan 1, 1996·Annual Review of Physiology·M R KaplanE Delpire
Oct 27, 2006·The Journal of Physical Chemistry. B·Naoki MimuraKazuhisa Murata
Oct 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·B ZhangC Tohyama
Feb 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A RossiM L Scarino
May 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I M BruggemanP J van Bladeren
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H F Bjerregaard

❮ Previous
Next ❯

Citations

Jan 27, 2007·Archives of Environmental Contamination and Toxicology·Stefanos Dailianis, Martha Kaloyianni
Dec 31, 2002·Toxicology·Alessandra GennariPilar Prieto
Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A Alvarez-BarrientosP Prieto
Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H F BjerregaardJ Vang
May 28, 2013·BioMed Research International·José L ReyesMaría Del Carmen Namorado
Jun 29, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Emmy Van KerkhoveQuirine Swennen
Nov 22, 2005·Biochimica Et Biophysica Acta·Marta Zuvić-ButoracJanko N Herak
Mar 7, 2000·Toxicology and Applied Pharmacology·B Faurskov, H F Bjerregaard
Jun 11, 2016·Toxicology and Applied Pharmacology·Xiaojiang TangXuefeng Ren
May 3, 2018·Biological Trace Element Research·Hongmei WangMing Ge
Mar 20, 2019·International Journal of Molecular Sciences·Rhiannon V McNeillJennifer Southgate

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.