Effect of cadmium on cytosine hydroxymethylation in gastropod hepatopancreas

Environmental Science and Pollution Research International
Dragos V NicaReinhard Stöger

Abstract

5-Hydroxymethylcytosine (5hmC) is an important, yet poorly understood epigenetic DNA modification, especially in invertebrates. Aberrant genome-wide 5hmC levels have been associated with cadmium (Cd) exposure in humans, but such information is lacking for invertebrate bioindicators. Here, we aimed to determine whether this epigenetic mark is present in DNA of the hepatopancreas of the land snail Cantareus aspersus and is responsive to Cd exposure. Adult snails were reared under laboratory conditions and exposed to graded amounts of dietary cadmium for 14 days. Weight gain was used as a sublethal endpoint, whereas survival as a lethal endpoint. Our results are the first to provide evidence for the presence of 5hmC in DNA of terrestrial mollusks; 5hmC levels are generally low with the measured values falling below 0.03%. This is also the first study to investigate the interplay of Cd with DNA hydroxymethylation levels in a non-human animal study system. Cadmium retention in the hepatopancreas of C. aspersus increased from a dietary Cd dose of 1 milligram per kilogram dry weight (mg/kg d. wt). For the same treatment, we identified the only significant elevation in percentage of samples with detectable 5hmC levels despite the lack ...Continue Reading

References

May 1, 1981·Bulletin of Environmental Contamination and Toxicology·L K RussellR P Botts
May 1, 1996·Bulletin of Environmental Contamination and Toxicology·W B Rabitsch
Jun 1, 1996·Ecotoxicology and Environmental Safety·R Laskowski, S P Hopkin
Aug 1, 1997·Archives of Environmental Contamination and Toxicology·A Gomot
Dec 18, 2002·Ecotoxicology and Environmental Safety·Michaël CoeurdassierPierre Marie Badot
Jul 11, 2006·Environment International·Frédéric GimbertPierre-Marie Badot
Aug 19, 2007·Environmental Pollution·Frédéric GimbertAnnette de Vaufleury
Mar 6, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Suphot WattanaphansakJohn Deen
Jan 1, 2008·Disability and Health Journal·Margaret Giannini
Jun 1, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Martina HöcknerReinhard Dallinger
Jul 21, 2011·Journal of Nucleic Acids·Weiwei Li, Min Liu
Nov 16, 2011·Nature Reviews. Genetics·Miguel R BrancoWolf Reik
Jan 14, 2012·Environmental Toxicology and Chemistry·Jessica A HeadNiladri Basu
Mar 3, 2012·Environmental Health Perspectives·Mohammad Bakhtiar HossainKarin Broberg
Apr 5, 2012·Current Medicinal Chemistry·B WangL Cai
May 30, 2012·Nature Reviews. Genetics·Peter A Jones
Mar 7, 2013·Nature Structural & Molecular Biology·Yehudit Bergman, Howard Cedar
May 7, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Isidoro FelicielloDurđica Ugarković
Jul 24, 2013·Marine Biotechnology·Guillaume RivierePascal Favrel
Aug 10, 2013·Methods in Molecular Biology·Leonid L Moroz, Andrea B Kohn
Oct 2, 2013·BMC Genomics·Pablo CingolaniDouglas M Ruden
Oct 31, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Alison P SandersRebecca C Fry
Dec 18, 2013·Environmental Science & Technology·Fabien PierronPatrice Gonzalez
May 23, 2014·Nature·Leonid L MorozAndrea B Kohn
May 27, 2014·Current Environmental Health Reports·T DaoWy Tang
Sep 27, 2014·The Science of the Total Environment·Jie ZhangHeqing Shen
Nov 9, 2014·G3 : Genes - Genomes - Genetics·Robert M ErdmannMary Gehring

❮ Previous
Next ❯

Citations

Jun 25, 2019·Environmental Toxicology and Chemistry·Maíra de Vasconcelos LimaAdriana Fontes
Apr 6, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Lisa MännerCarola Greve
Apr 12, 2021·Environmental Science and Pollution Research International·Maxime LouzonNicolas Capelli
Nov 5, 2019·Gene·Manon FalletCéline Cosseau
Mar 29, 2019·Chemical Research in Toxicology·Jun XiongYu-Qi Feng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.