PMID: 22568336May 10, 2012Paper

Effect of calcitonin gene-related peptide on proliferation and migration of human umbilical vein endothelial cells

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Yonghua TuoDan Jin

Abstract

Tissue engineered bone implanted with sensory nerve can effectively promote angiogenesis and repair of bone defects. To investigate the effects of calcitonin gene-related peptide (CGRP) on proliferation and migration of human umbilical vein endothelial cells (HUVECs) for further revealing the mechanism of tissue engineered bone implanted with sensory nerve promoting angiogenesis. HUVECs were collected from human umbilical core, and identified through von Willebrand factor (vWF) and CD31 immunofluorescence. The HUVECs were treated with CGRP and were divided into 6 groups according to CGRP concentration: group A (0 mol/L), group B (1 x 10(-12) mol/L), group C (1 x 10(-11) mol/L), group D (1 x 10(-10) mol/L), group E (1 x 10(-9) mol/L), and group F (1 x 10(-8) mol/L). The expression of the CGRP1 receptor (CGRP1R) was observed in HUVECs by cell immunofluorescence. The growth rate of HUVECs was detected throug AAlarmarBlue at 1, 2, 3, 4, and 5 days. Transwell chamber was used to detect the ability of cell migration. ELISA assay was used to detect the vascular endothelial growth factor (VEGF) secretion and the protein expression of focal adhesion kinase (FAK) was examined using Western blot. HUVECs were identified through morphology,...Continue Reading

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