Effect of calcium glycerophosphate on demineralization in an in vitro biofilm model

Caries Research
R J M Lynch, J M ten Cate

Abstract

The aim was to investigate the anti-caries properties of calcium glycerophosphate (CaGP) using an in vitro bacterial flow cell model. Four flow cells, inoculated from a chemostat containing a seven-organism bacterial consortium, were pulsed with sucrose twice daily, to provide an acidic challenge and pH-cycling conditions. Blocks of enamel and dentine were mounted in each flow cell. In a study on the effect of CaGP concentration, CaGP was pulsed into three of the flow cells, at the same time as the sucrose, to give concentrations of 0.10, 0.25 and 0.50%. Water was pulsed into the fourth flow cell with the sucrose. Microradiography revealed a significant dose response of decreasing demineralization as CaGP concentration increased. Reductions at 0.25 and 0.5% were significant when compared to the control. A second study investigated the effect of timing of CaGP pulsing, relative to sucrose, on enamel and dentine demineralization. CaGP (flow cell concentration 0.2%), was pulsed 1 h before, during or 1 h after the sucrose pulse; a water control was employed. In enamel, pulsing CaGP before the sucrose reduced demineralization significantly compared to concurrent pulsing, which in turn gave a significant reduction compared to pulsing...Continue Reading

References

Jul 6, 1985·British Dental Journal
Dec 1, 1966·Australian Dental Journal·B LilienthalD H Napper
Mar 1, 1971·Journal of Dental Research·G NikiforukG W Thompson
Jan 1, 1983·Archives of Oral Biology·W H Van Palenstein HeldermanJ H Huis in 't Veld
Feb 1, 1996·The Journal of Applied Bacteriology·D J BradshawD Cummins
Apr 12, 2001·Journal of Applied Microbiology·R J HodgsonC Allison

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Citations

Sep 26, 2006·Odontology·Jacob M ten Cate
Oct 4, 2011·PloS One·Sebastian SchlaferBente Nyvad
Nov 19, 2013·Clinical Oral Investigations·Ana Carolina Soares Fraga ZazeAlberto Carlos Botazzo Delbem
Mar 11, 2016·Dental Research Journal·Satyawan Gangaramji DamleSimranjeet Kaur
Mar 23, 2010·Dental Materials : Official Publication of the Academy of Dental Materials·Richard P RusinDavid H Pashley
Jul 16, 2015·Brazilian Oral Research·Thiago Saads CarvalhoMarcelo José Strazzeri Bönecker
May 12, 2017·Brazilian Dental Journal·Gabriela D CanaliEvelise M Souza
Oct 17, 2017·Applied and Environmental Microbiology·Xia LuoKristen L Jellison
Oct 14, 2009·Dental Materials Journal·Kenichi TajimaJunji Tagami
Apr 6, 2018·Caries Research·Audrey C C HollandersMarie-Charlotte D N J M Huysmans
Jun 30, 2018·Antibiotics·Gabriela Lopes FernandesDebora Barros Barbosa
Feb 28, 2019·Journal of Applied Oral Science : Revista FOB·Sâmia Sass SantosDenise Pedrini
Nov 6, 2018·Journal of Dental Research·S G DashperE C Reynolds
Mar 7, 2021·Microorganisms·María Carmen SánchezMariano Sanz
Nov 16, 2012·The Journal of Contemporary Dental Practice·Shiny BenjaminMohan Thomas Nainan

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