Sep 1, 1989

Effect of capsule permeability on growth of CHO cells in Eudragit RL microcapsules: use of FITC-dextran as a marker of capsule quality

Biomaterials
R L Broughton, M V Sefton

Abstract

Chinese hamster ovary fibroblast cells were encapsulated in Eudragit RL by interfacial precipitation. After encapsulation, they grew to fill the capsules, eventually growing outside the capsules as well. By encapsulating FITC-dextran (MW 150,000) along with the cells, it was possible to show that cell growth was faster in those capsules which lost fluorescence soon after encapsulation. These data were interpreted to signify that the intact Eudragit RL capsule membrane offered significant diffusion resistance to vital nutrients or particular metabolites, slowing or even preventing growth.

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References

  • References3
  • Citations7

Citations

Mentioned in this Paper

Ovarian Diseases
Cricetulus
Chinese Hamster
Microbial Anatomical Capsule Structure
Both Ovaries
Eudragit RL
Ovary
Microcapsules Drug Delivery System
Chinese Hamster Ovary Cell
Permeability

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