Effect of carboidiimide on thermal denaturation temperature of dentin collagen

Dental Materials : Official Publication of the Academy of Dental Materials
Milena CadenaroLorenzo Breschi

Abstract

1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) has been shown to cross-link dentin type I collagen. Increased cross-linking usually elevates the glass transition temperature of polymers. The aim of this study was to evaluate the cross-linking reaction promoted by EDC in different aqueous concentrations by measuring the thermal denaturation temperature (Td) of human dentin collagen. The Td of dehydrated collagen and of insoluble dentin matrix collagen immersed in 0.5M or 1M EDC aqueous solution for different treatment times was obtained using a Differential Scanning Calorimeter (DSC). Specimens were also analyzed by Energy Dispersive X-Ray Spectroscopy. EDC-treated dentin collagen showed a significantly higher Td than the untreated specimens when immersed in either 0.5M EDC or 1M EDC for 10min or longer (p<0.05). EDC-treated dentin collagen showed an increase of sulfur and chloride, not detectable in EDC-untreated dentin specimens. Conversely, the relative amount of carbon, nitrogen and oxygen was not modified by treatments. EDC-treated dentin collagen showed a higher Td than the untreated control at all tested concentrations and immersion times. A higher Td can be considered an indirect indicator of a more r...Continue Reading

References

Jan 1, 1995·Bioconjugate Chemistry·N Nakajima, Y Ikada
Sep 2, 1998·Journal of Structural Biology·D J Prockop, A Fertala
Mar 14, 2003·Journal of Biomedical Materials Research. Part a·Bo HanMarcel E Nimni
Apr 23, 2003·Journal of Inorganic Biochemistry·Nishtar Nishad FathimaBalachandran Unni Nair
May 8, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P GarneroJ M Delaissé
Jul 17, 2004·The EMBO Journal·Linda ChungHideaki Nagase
Sep 18, 2004·International Journal of Biological Macromolecules·Nishtar Nishad FathimaThirumalachari Ramasami
Jun 13, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ana Karina B Bedran-RussoMitsuo Yamauchi
Jun 24, 2006·Journal of Endodontics·Steven R ArmstrongDavid H Pashley
Apr 20, 2007·Dental Materials : Official Publication of the Academy of Dental Materials·Lorenzo BreschiElettra De Stefano Dorigo
Dec 29, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ana Karina B Bedran-RussoKlaus J Miescke
Mar 13, 2008·Journal of Biomedical Materials Research. Part a·Annalisa MazzoniLorenzo Breschi
Feb 23, 2010·Journal of Endodontics·Ivarne L TersariolLeo Tjäderhane
Mar 20, 2010·Dental Materials : Official Publication of the Academy of Dental Materials·Lorenzo BreschiDavid H Pashley
May 18, 2010·Dental Materials : Official Publication of the Academy of Dental Materials·Jongryul KimDavid H Pashley
Jun 5, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ana Karina B Bedran-RussoCarina S Castellan
Oct 12, 2010·International Journal of Biological Macromolecules·N Nishad FathimaR Miller
Nov 30, 2010·Dental Materials : Official Publication of the Academy of Dental Materials·David H PashleyArzu Tezvergil-Mutluay
Jan 7, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Changqi Xu, Yong Wang
Jan 11, 2011·Journal of Dentistry·Annalisa MazzoniLorenzo Breschi
Jul 26, 2011·Journal of the Mechanical Behavior of Biomedical Materials·Carina Strano CastellanPatrícia Nóbrega Rodrigues Pereira
Sep 24, 2011·Journal of Dental Research·A CovaA Mazzoni
Nov 8, 2011·Journal of Dental Research·A Tezvergil-MutluayD H Pashley
Dec 17, 2011·Journal of Dental Research·J De MunckB Van Meerbeek
Mar 15, 2012·Acta Biomaterialia·Luiz E BertassoniMichael V Swain
Aug 21, 2012·Dental Materials : Official Publication of the Academy of Dental Materials·Leo TjäderhaneDavid H Pashley
Nov 6, 2012·International Journal of Biological Macromolecules·Marta Safandowska, Krystyna Pietrucha
Aug 7, 2013·Dental Materials : Official Publication of the Academy of Dental Materials·Annalisa MazzoniLorenzo Breschi
Dec 18, 2013·Dental Materials : Official Publication of the Academy of Dental Materials·Débora L S ScheffelDavid H Pashley
Dec 18, 2013·Journal of Dental Research·A MazzoniL Breschi
Jan 24, 2015·Dental Materials : Official Publication of the Academy of Dental Materials·Kelli A AgeeDavid H Pashley
May 20, 2015·Journal of Dentistry·Débora Lopes Salles ScheffelJosimeri Hebling

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Citations

Oct 24, 2017·Calcified Tissue International·J CaiM F Burrow
Sep 16, 2020·Materials·Cheol-Hwi Kim, Tae-Hyung Kim
Mar 2, 2021·Cellular and Molecular Bioengineering·İrem Deniz DermanMurat Kazanci
Sep 26, 2017·Dental Materials : Official Publication of the Academy of Dental Materials·Zihou ZhangDwayne Arola
Nov 29, 2017·Dental Materials : Official Publication of the Academy of Dental Materials·Annalisa MazzoniLorenzo Breschi

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