PMID: 8580643Jan 1, 1995Paper

Effect of carbon:nitrogen ratio on kinetics of phenol biodegradation by Acinetobacter johnsonii in saturated sand

Biodegradation
B L HoyleJ L Darby

Abstract

In polluted soil or ground water, inorganic nutrients such as nitrogen may be limiting, so that Monod kinetics for carbon limitation may not describe microbial growth and contaminant biodegradation rates. To test this hypothesis we measured 14CO2 evolved by a pure culture of Acinetobacter johnsonii degrading 120 micrograms 14C-phenol per ml in saturated sand with molar carbon:nitrogen (CN) ratios ranging from 1.5 to 560. We fit kinetics models to the data using non-linear least squares regression. Phenol disappearance and population growth were also measured at CN1.5 and CN560. After a 5- to 10-hour lag period, most of the 14CO2 evolution curves at all CN ratios displayed a sigmoidal shape, suggesting that the microbial populations grew. As CN ratio increased, the initial rate of 14CO2 evolution decreased. Cell growth and phenol consumption occurred at both CN1.5 and CN560, and showed the same trends as the 14CO2 data. A kinetics model assuming population growth limited by a single substrate best fit the 14CO2 evolution data for CN1.5. At intermediate to high CN ratios, the data were best fit by a model originally formulated to describe no-growth metabolism of one substrate coupled with microbial growth on a second substrate. W...Continue Reading

References

Apr 1, 1979·Applied and Environmental Microbiology·J T Dibble, R Bartha
Apr 1, 1978·Journal of Pharmacokinetics and Biopharmaceutics·K YamaokaT Uno
Jan 1, 1988·Advances in Applied Microbiology·W C Ghiorse, J T Wilson
Aug 1, 1985·Applied and Environmental Microbiology·S K SchmidtM Alexander
Nov 1, 1972·Canadian Journal of Microbiology·R D MegeeH M Tsuchiya
Jun 1, 1984·Applied and Environmental Microbiology·S Simkins, M Alexander
Jun 1, 1981·Regulatory Toxicology and Pharmacology : RTP·H Babich, D L Davis
May 1, 1983·Applied and Environmental Microbiology·J A Robinson, J M Tiedje
Jan 1, 1984·Applied and Environmental Microbiology·W Brunner, D D Focht
Oct 1, 1985·Applied and Environmental Microbiology·D D Focht, W Brunner
Feb 1, 1986·Applied and Environmental Microbiology·D F Paris, J E Rogers
Jun 1, 1990·Applied and Environmental Microbiology·T F HessB Howe

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Citations

Nov 12, 2005·Applied Microbiology and Biotechnology·Anli GengLeslie Ching Thin Loke
Nov 25, 1998·Research in Microbiology·C Barberio, R Fani
Sep 9, 2008·The Science of the Total Environment·Alfredo Pérez-de-MoraMichael Schloter
Sep 24, 2004·Journal of Biotechnology·Manfred ZinnThomas Egli
Jan 31, 2009·FEMS Microbiology Reviews·Janmejay PandeyRakesh K Jain
Sep 13, 2007·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·M Z AlamP Jamal
Mar 17, 2007·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Zahangir AlamParveen Jamal

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