Effect of carotenoids against genotoxicity of diethylnitrosamine on rat hepatocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
T García-GascaE González de Mejía

Abstract

Carotenoids have been considered as special nutrients due to their biological activity as provitamin A compounds, and because of their natural antioxidant and anticarcinogenic properties. The main objective of this study was to evaluate the protective effect of carotenoids against the genotoxic cellular damage induced by diethylnitrosamine (DEN), a potent hepatocarcinogen. Normal and freshly isolated hepatocytes were cultured as the biological system. Concentrations of 2.5 and 5 mum DEN caused 1.3 and 2.0 times more DNA T(3)H incorporation, respectively, when compared with control cells. Pure carotenoids, beta-carotene (50 mum), lutein (1 mum) and a carotenoid extract from green peppers (1 mum eq. lutein) were used as functional nutrients to protect the cells. All the carotenoids studied prevented the genotoxic damage caused by 2.5 mum DEN. When 5 mum DEN was used, only beta-carotene and the pepper extract inhibited the damage up to 30-40%. Carotenoids provide a dose-dependent protective effect against DNA damage induced by DEN in isolated hepatocytes.

References

Nov 15, 1976·Experientia·D A Young
Jan 1, 1990·Critical Reviews in Food Science and Nutrition·M Namiki
Jan 1, 1990·Mutation Research·M D WatersH E Brockman
Jan 1, 1987·Cancer Metastasis Reviews·W Lijinsky
Jan 1, 1981·Journal of Cancer Research and Clinical Oncology·P D Lotlikar
Jun 1, 1995·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·S TomaR Palumbo
Jan 1, 1993·Molecular Aspects of Medicine·N I Krinsky
Sep 1, 1993·Canadian Journal of Physiology and Pharmacology·A DavisonB Dunn
Jan 26, 1996·Science·K Peterson
Sep 1, 1996·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·P N Magee

❮ Previous
Next ❯

Citations

May 22, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J I Pérez-CarreónS Villa-Treviño
Jan 23, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M González-AvilaS Villa-Treviño
Aug 30, 2008·Regulatory Toxicology and Pharmacology : RTP·A VikramG B Jena
Dec 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Reyes-LópezM de la Garza

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.