Effect of catalase and thioredoxin addition to sperm incubation medium before in vitro fertilization on sperm capacity to support embryo development

Fertility and Sterility
Y Kuribayashi, C Gagnon

Abstract

To determine whether addition of catalase and thioredoxin to sperm incubation medium before IVF improves sperm potential to support embryo development. CD-1 mouse spermatozoa were preincubated without or with catalase or thioredoxin for 1 hour before IVF, and sperm motility parameters, fertilization rate, and embryo development were determined. A conventional laboratory setting. Mice were superovulated with pregnant mare serum gonadotropin and hCG. Eggs in cumulus oophorus were collected and used for fertilization with epididymal spermatozoa. Sperm motility parameters, fertilization rate, and embryo development. Sperm motility parameters and fertilization rates were not affected by catalase treatment. However, the rates of blastocyst and hatching blastocyst formation when catalase-treated (16 micrograms/mL) spermatozoa were used were significantly higher than those observed with nontreated spermatozoa (44% versus 24%, 31% versus 2%, respectively). Addition of thioredoxin to preincubation media did not affect the percentage of motility and the fertilization rate but increased the rate of blastocyst formation to an extent similar to that triggered by catalase (2.7- and 3.3-fold, respectively). These results suggest that H2O2 prod...Continue Reading

Citations

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