Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS

Journal of Animal Science and Biotechnology
K HaziakD Tomaszewska-Zaremba

Abstract

Immune stress induced by lipopolysaccharide (LPS) influences the gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) secretion. Presence of LPS interacting Toll-like receptor (TLR) 4 in the hypothalamus may enable the direct action of LPS on the GnRH/LH secretion. So, the aim of the study was to investigate the influence of intracerebroventricular (icv) injection of TLR4 antagonist on GnRH/LH secretion in anestrous ewes during LPS-induced central inflammation. Animals were divided into three groups icv-treated with: Ringer-Locke solution, LPS and TLR4 antagonist followed by LPS. It was demonstrated that TLR4 antagonist reduced LPS-dependent suppression of GnRH gene expression in the preoptic area and in the medial basal hypothalamus, and suppression of receptor for GnRH gene expression in the anterior pituitary gland. It was also shown that TLR4 antagonist reduced suppression of LH release caused by icv injection of LPS. Central administration of LPS stimulated TLR4 gene expression in the medial basal hypothalamus. It was indicated that blockade of TLR4 prevents the inhibitory effect of centrally acting LPS on the GnRH/LH secretion. This suggests that some negative effects of bacterial infection on the hypothalamic-p...Continue Reading

References

Jan 1, 1988·Histochemistry·M CaldaniM P Dubois
Feb 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E T RietschelF Di Padova
Jul 1, 1996·Journal of Neurology, Neurosurgery, and Psychiatry·K FassbenderM Hennerici
Mar 20, 1998·European Journal of Endocrinology·F DadounC Oliver
Oct 24, 1998·Domestic Animal Endocrinology·P S KalraS P Kalra
Mar 28, 2001·Annals of the New York Academy of Sciences·S M McCannV Rettori
Oct 30, 2001·International Journal of Medical Microbiology : IJMM·C J Kirschning, S Bauer
Dec 26, 2001·The Journal of Biological Chemistry·Guolong Zhang, Sankar Ghosh
Nov 26, 2002·Journal of Neuroimmunology·John J HaddadBared Safieh-Garabedian
Feb 13, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·Hilary DobsonRobert Smith
Dec 15, 2004·Microbes and Infection·Katherine A FitzgeraldDouglas T Golenbock
Feb 18, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sumana Chakravarty, Miles Herkenham
Sep 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carolyn S JackJack P Antel
May 6, 2006·The Journal of Allergy and Clinical Immunology·Tsuneyasu Kaisho, Shizuo Akira
Aug 1, 2006·Neurologic Clinics·Robert Dantzer
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hiroaki MitsuzawaYoshio Kuroki
Oct 6, 2007·Immunologic Research·Theresa L Gioannini, Jerrold P Weiss
Nov 14, 2008·Veterinary Immunology and Immunopathology·Jung-Su ChangDavid M Haig
Jan 16, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Marciane MilanskiLício A Velloso
Apr 9, 2009·Reproductive Biology·Dorota Tomaszewska-Zaremba, Andrzej Herman
Sep 9, 2009·Brain, Behavior, and Immunity·William A Banks, Sandra M Robinson
Mar 30, 2010·Reproduction in Domestic Animals = Zuchthygiene·A P HermanDorota Tomaszewska-Zaremba
Apr 30, 2010·Animal Reproduction Science·Andrzej Przemysław Herman, Dorota Tomaszewska-Zaremba
May 21, 2011·Reproduction in Domestic Animals = Zuchthygiene·A P HermanD Tomaszewska-Zaremba

❮ Previous
Next ❯

Citations

Jan 18, 2020·International Journal of Molecular Sciences·Klaudia BarabásIstván M Ábrahám
Apr 29, 2021·Inflammation·Talha UmarGanzhen Deng

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

BestKeeper
STATISTICA
Primer

Related Concepts

Related Feeds

Aphasia

Aphasia affects the ability to process language, including formulation and comprehension of language and speech, as well as the ability to read or write. Here is the latest research on aphasia.