Effect of CD4+ T lymphocyte depletion on resistance of Gulf Coast Native lambs to Haemonchus contortus infection

Veterinary Parasitology
M T PeñaD W Horohov

Abstract

It has been reported that CD4(+) T lymphocytes are important in acquired immunity to gastrointestinal nematode infection. Whether these lymphocytes are also involved in the immune response of naturally resistant Gulf Coast Native (GCN) sheep to Haemonchus contortus infection remains to be defined. The objective of this study was to determine the role of CD4(+) T lymphocytes in this resistance. Ten GCN lambs were randomly assigned to a control (n=5) or a treatment (n=5) group. The treatment consisted of a series of IV injections with mouse anti-ovine CD4(+) T lymphocyte monoclonal antibodies for a period of 3 weeks. After the second treatment, all lambs were experimentally infected with 10,000 H. contortus infective larvae by oral inoculation. All lambs were monitored for fecal egg counts, blood packed cell volumes, white blood cell differential counts and serum antibody responses on a weekly basis. Fluorescence-activated cell sorter (FACS) analysis was done biweekly to enumerate CD4(+) T lymphocytes in peripheral blood. Necropsies were performed at the end of the study and 10% of the contents of the gastrointestinal tract were preserved for nematode enumeration and identification. Also at necropsy, mesenteric lymph nodes were e...Continue Reading

References

Feb 1, 1985·The Journal of Experimental Medicine·D Wofsy, W E Seaman

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Citations

Mar 20, 2013·BioMed Research International·Fernando Alba-Hurtado, Marco Antonio Muñoz-Guzmán
Feb 23, 2013·Veterinary Research Communications·Virginia M VenturinaJohn Hopkins
Apr 23, 2010·Veterinary Parasitology·L G ZarosL L Coutinho
Feb 26, 2008·Journal of Comparative Pathology·J PérezA Martínez-Moreno
May 20, 2015·Veterinary Parasitology·Jesica R JacobsScott A Bowdridge
Jul 1, 2020·Parasite Immunology·Elizabeth ShepherdScott A Bowdridge
Nov 3, 2020·Developmental and Comparative Immunology·Alexandria GillespieCynthia L Baldwin

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