Effect of CEACAM-1 knockdown in human colorectal cancer cells

Oncology Letters
Zhong-Min HanYong-Wu Sun

Abstract

Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1) is the major antigen of the CD66 cluster of granulocyte differentiation antigens. The present study aimed to assess the biological function of CEACAM-1 on the growth of human colorectal cancer (CRC) cells in vitro. Treatment of cultured CRC HCT-8 cells with CEACAM-1-specific siRNA successfully downregulated CEACAM-1 expression by 61% compared with control cells. The effects of CEACAM-1 downregulation on HCT-8 cell proliferation and apoptosis were then assessed via Cell Counting kit-8 assay and flow cytometry, respectively. The results demonstrated that siRNA-induced CEACAM-1 downregulation significantly inhibited proliferation and increased apoptosis, but had no significant effect on cell cycle progression in HCT-8 cells. Together, these results suggest that CEACAM-1 activity is critical to CRC growth, and thus, CEACAM-1 may be a promising therapeutic target for the treatment of CRC.

References

Sep 1, 1994·Human Pathology·M Pignatelli, C J Vessey
Aug 15, 2001·Experimental Cell Research·N BeaucheminW Zimmermann
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 16, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Anka ThiesUdo Schumacher
Jan 5, 2007·BMC Cancer·Rosalyn D BlumenthalDavid M Goldenberg
Apr 21, 2007·Pancreas·Diane M SimeoneCraig D Logsdon
Jan 25, 2008·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Sebastian DangoBernward Passlick
Mar 19, 2013·Experimental Cell Research·Yun Li, John E Shively
Aug 2, 2013·Cancer Metastasis Reviews·Nicole Beauchemin, Azadeh Arabzadeh
Jul 30, 2016·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Tiziana RossoCarlo La Vecchia
Aug 4, 2016·Chinese Journal of Cancer·Wanqing ChenSiwei Zhang

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Methods Mentioned

BETA
protein assay
electrophoresis
flow cytometry

Software Mentioned

ImageJ
Modfit

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