Effect of cell density on energy-dependent calcium uptake by Balb/c 3T3 membranes is independent of protein synthesis and attachment to substratum

Journal of Cellular Physiology
L Moore, I Pastan


Membranes isolated from subconfluent cultures of Balb/c 3T3 cells have low energy-dependent calcium uptake activity. Replating confluent cells at low density results in a prompt fall of energy-dependent calcium uptake by membrane fractions. The level to which uptake activity falls is a function of the density at which the cells are plated (Moore and Pastan, '77b). To determine if regulation of energy-dependent uptake of calcium by membrane fractions is dependent upon attachment to a substrate and to further characterize conditions that regulate the process, we examined calcium uptake activity of membranes isolated from cells in suspension. With cells in suspension energy-dependent calcium uptake activity of isolated membranes falls promptly if cells are diluted to a low density (less than 10(5) cells/ml) and is a function of cell density. When cells in suspension at low cell densities are concentrated to high cell densities (greater than 2 x 10(6) cells/ml), calcium uptake activity of the isolated membrane fraction is increased as a function of cell density. These changes of membrane calcium uptake activity occur promptly and do not require protein synthesis.


Jun 1, 1988·Journal of Cellular Physiology·E M SchaeferT J Moehring


Oct 1, 1979·Journal of Cellular Physiology·L Moore, I Pastan
Aug 22, 1977·Biochemical and Biophysical Research Communications·R M Gopinath, F F Vincenzi
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A L Boynton, J F Whitfield
Jun 1, 1973·Experimental Cell Research·M H GailC S Thompson
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·S D Balk

Related Concepts

Mice, Inbred BALB C
Cell Density
Subcellular Fractions
Hydrogen-Ion Concentration
Plasma Membrane

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