Effect of cell geometry in the evaluation of erythrocyte viscoelastic properties.

Physical Review. E
Fran GómezNathan B Viana

Abstract

The red blood cell membrane-cytoskeleton is a complex structure mainly responsible for giving the cell rigidity and shape. It also provides the erythrocyte with the ability to pass through narrow capillaries of the vertebrate blood circulatory system. Although the red blood cell viscoelastic properties have been extensively studied, reported experimental data differ by up to three orders of magnitude. This could be attributed to the natural cell variability, to the different techniques employed, and also to the models used for the cell response, which are highly dependent on cell geometry. Here, we use two methodologies based on optical tweezers to investigate the viscoelastic behavior of healthy human red blood cells, one applying small cell deformations (microrheology) and another imposing large deformations (tether extraction). We also establish a defocusing microscopy-based method to characterize the cell geometry and thus the erythrocyte form factor, an essential parameter that allows comparisons among the viscoelastic properties at different conditions. Moreover, for small deformations, a soft glassy rheology model is used to discuss the results, while for large deformations two surface shear moduli and one surface viscos...Continue Reading

References

Apr 1, 1979·Biophysical Journal·R M HochmuthE A Evans
Apr 1, 1979·Biophysical Journal·R Waugh, E A Evans
Jan 1, 1992·Biophysical Journal·D A Berk, R M Hochmuth
Nov 1, 1973·Zeitschrift Für Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie·W Helfrich
May 1, 1995·Annals of Biomedical Engineering·R E Waugh, R G Bauserman
Jan 1, 1996·Biophysical Journal·F M HochmuthM P Sheetz
Jun 27, 2000·Biophysical Journal·W RawiczE Evans
Feb 28, 2002·Biophysical Journal·Zhiwei LiWilliam E Brownell
May 15, 2002·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Thomas R PowersRaymond E Goldstein
Jun 6, 2003·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·U AgeroO N Mesquita
Dec 18, 2004·Microscopy Research and Technique·U AgeroO N Mesquita
Apr 18, 2006·Biophysical Journal·José Coelho NetoOscar N Mesquita
Oct 10, 2006·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Martial BallandFrançois Gallet
Apr 13, 2007·American Journal of Physiology. Cell Physiology·Marina Puig-de-Morales-MarinkovicSubra Suresh
Jun 1, 2007·Nature·Xavier TrepatJeffrey J Fredberg
Dec 10, 2007·Optics Express·M Shahrooz AminGabriel Popescu
Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Timo BetzCécile Sykes
May 21, 2010·Biophysical Journal·Dmitry A FedosovGeorge Em Karniadakis
Aug 21, 2010·Biomechanics and Modeling in Mechanobiology·Thomas Klöppel, Wolfgang A Wall
Jul 5, 2011·Biophysical Journal·B PontesH M Nussenzveig
Oct 19, 2011·Biophysical Journal·John P HalePeter G Petrov
Nov 22, 2011·Biophysical Journal·Andrea NansDavid L Stokes
Jul 12, 2013·PloS One·Bruno PontesH Moysés Nussenzveig
Jan 1, 2014·Frontiers in Physiology·Amr AlaargRichard van Wijk
Jun 25, 2016·BMC Biophysics·Yareni A AyalaH Moysés Nussenzveig
Dec 31, 2016·Experimental Cell Research·Yareni A AyalaH Moysés Nussenzveig
Apr 13, 2017·Scientific Reports·S KapishnikovJ Als-Nielsen

❮ Previous
Next ❯

Citations

Jun 3, 2021·Life·Andrea CugnoKlaus Ley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.