Effect of cell polarization and differentiation on entry of Listeria monocytogenes into the enterocyte-like Caco-2 cell line.

Infection and Immunity
J L Gaillard, B B Finlay

Abstract

The entry of Listeria monocytogenes into the enterocyte-like Caco-2 cell line was studied as a function of cell polarization and differentiation. L. monocytogenes entered through the entire surface of nonpolarized cells and, predominantly, through the basolateral surface of polarized cells based on the following observations: (i) sites of L. monocytogenes invasion paralleled the distribution of the transferrin receptor, a well-known basolateral marker of polarization; (ii) numbers of internalized bacteria decreased dramatically when Caco-2 monolayers cultured beyond confluency were used (about 0.1% of the inoculated bacteria versus 1 to 2% with nonconfluent monolayers); and (iii) L. monocytogenes entry into postconfluent monolayers was greatly enhanced by treating cells with Ca(2)+ -free medium, a procedure that disrupts intercellular junctions and thus exposes the basolateral surface to bacteria. Ethylene glycol-bis (beta-aminoethyl ether)-N, N, N',N' -tetraacetic acid (EGTA) had contradictory effects on L. monocytogenes entry as this reagent opened intercellular junctions but inhibited binding and internalization of bacteria. Finally, the role of the inlAB locus in L. monocytogenes entry was confirmed because and inlAB mutant...Continue Reading

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Citations

Jan 19, 1999·Annual Review of Cell and Developmental Biology·S Dramsi, P Cossart
Sep 7, 2001·Annual Review of Microbiology·B I KazmierczakJ N Engel
Jan 27, 2004·Infection and Immunity·Jennifer L WamplerArun K Bhunia
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Jul 24, 2004·Infection and Immunity·Eliane MilohanicJean-Louis Gaillard
Apr 7, 2010·Applied and Environmental Microbiology·Damien BalestrinoAlejandro Toledo-Arana
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Dec 4, 2003·The Journal of Nutrition·Marianna RoselliElena Mengheri
Sep 8, 2017·Molecular Biology of the Cell·Fabian E OrtegaJulie A Theriot

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