Effect of cellular level of FliK on flagellar hook and filament assembly in Salmonella typhimurium

Journal of Molecular Biology
K MuramotoR M Macnab

Abstract

Frameshift mutations in the fliK gene of Salmonella result in abnormal elongation of the hook and the failure to assemble filament (polyhook phenotype). Second-site suppressor mutations restore filament assembly, but the cells often remain defective in hook-length control (polyhook-filament phenotype). Where the suppressor mutations are intragenic, the second mutation restores the original frame, generating a region of frameshifted sequence, but restoring the natural C terminus. Some of these frameshifted sequences contain a UGA (opal) termination codon. These cells have few flagella and swarm poorly. We suspected that readthrough of UGA by tRNATrp might be the reason for the partial function. When the UGA codon was changed to the Trp codon UGG, flagellar assembly and function were restored to wild-type levels. Conversely, underexpression of the wild-type fliK gene, achieved by changing the sole Trp codon in the sequence (Trp271) to UGA, decreased both the number of flagella and the ability to swarm. These results validate the readthrough hypothesis and indicate that low levels of FliK sustain some degree of flagellation and motility. At low levels of FliK, most flagella had polyhooks. With increasing amounts, the morphology pr...Continue Reading

References

Jan 1, 1976·Journal of Bacteriology·H KagawaN Takahashi
Feb 1, 1978·Journal of Bacteriology·T SuzukiS Yamaguchi
Oct 1, 1979·Journal of Bacteriology·K KutsukakeT Iino
Jul 20, 1992·Journal of Molecular Biology·T KuboriS Aizawa
Sep 1, 1989·Microbiological Reviews·J Parker
Mar 26, 1973·Archiv für Mikrobiologie·J Patterson-DelafieldS Yamaguchi
Jan 1, 1974·Journal of Supramolecular Structure·T Iino
Jun 14, 1971·Journal of Molecular Biology·D Hirsh, L Gold
Feb 1, 1981·Journal of Bacteriology·T Suzuki, Y Komeda
Dec 1, 1981·Journal of Bacteriology·T Suzuki, T Iino
Jan 5, 1993·Journal of Molecular Biology·D G MorganD J DeRosier

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Citations

Jul 14, 2000·Journal of Bacteriology·T MinaminoR M Macnab
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Partho Ghosh
Jun 2, 2004·Journal of Molecular Microbiology and Biotechnology·Ronald J Limberger
Apr 5, 2008·PLoS Pathogens·Robert J CainVassilis Koronakis
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·T KuboriJ E Galán
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Daniel H Wee, Kelly T Hughes
Jul 28, 2004·Journal of Molecular Biology·Tohru MinaminoKeiichi Namba
Apr 26, 2007·Protein Science : a Publication of the Protein Society·Richard C WatersKieran A Ryan
May 16, 2006·Canadian Journal of Microbiology·Claudia Goyer, Matthias S Ullrich
Apr 9, 2005·Molecular Microbiology·Takanori HiranoShin-Ichi Aizawa
Oct 22, 2008·Molecular BioSystems·Tohru MinaminoKeiichi Namba
Feb 6, 2010·Molecular Microbiology·Marc ErhardtKelly T Hughes
Aug 24, 2005·The Journal of Biological Chemistry·Manuelle QuinaudIna Attree
Jan 19, 1999·Annual Review of Microbiology·K T Hughes, K Mathee
Nov 14, 2002·Annual Review of Genetics·Nyles W Charon, Stuart F Goldstein
May 6, 2003·Annual Review of Microbiology·Robert M Macnab
Jun 10, 2011·The EMBO Journal·Marc ErhardtKelly T Hughes
Jan 21, 2000·FEMS Microbiology Reviews·L A Fernández, J Berenguer
Oct 18, 2005·The Journal of Biological Chemistry·Roma KenjaleAriel Blocker
Apr 27, 2017·Current Microbiology·Haiying Gu

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