Effect of cessation of phospholipid synthesis on the synthesis of a specific membrane-associated bacteriophage protein in Escherichia coli.

Journal of Bacteriology
J S Cashman, R E Webster

Abstract

The major coat protein of the bacteriophage f1 is synthesized during infection of Escherichia coli and becomes tightly associated with the host membrane. This synthesis was studied in conjunction with the strain BB26-36, a mutant defective in phospholipid synthesis, to investigate basic questions concerning membrane protein and phospholipid synthesis. Coat protein synthesis is decreased in the absence of net phospholipid synthesis. The coat protein produced under these conditions is still found tightly associated with the membrane. Resumption of phospholipid synthesis leads to an increase in the synthesis and accumulation of the coat protein. Therefore, a correlation between coat protein and phospholipid synthesis seems to exist. However, the packaging of phage deoxyribonucleic acid into phage particles proceeds in the absence of phospholipid synthesis, and the number of phage particles produced appears to depend only on the amount of coat protein in the membrane.

References

Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·W Wickner
Dec 1, 1975·CRC Critical Reviews in Microbiology·D T Denhardt
Feb 18, 1972·Science·S J Singer, G L Nicolson
Dec 5, 1974·Journal of Molecular Biology·G PieczenikH D Robertson
Sep 27, 1972·Nature: New Biology·C MilsteinM B Mathews
Jan 1, 1972·Journal of Supramolecular Structure·H SmilowitzP W Robbins
Feb 5, 1974·Journal of Molecular Biology·D PrattJ Griffith
Oct 14, 1968·Journal of Molecular Biology·D Pratt, W S Erdahl
Jun 1, 1969·Bacteriological Reviews·D A Marvin, B Hohn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.