Effect of Chaiqinchengqi decoction on sarco/endoplasmic reticulum Ca2+-ATPase mRNA expression of pancreatic tissues in acute pancreatitis rats.

World Journal of Gastroenterology : WJG
Ping XueMei-Hua Wan

Abstract

To investigate the effect of Chaiqinchengqi decoction (CQCQD) on sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) mRNA expression of pancreatic tissues in acute pancreatitis (AP) rats. Thirty Sprague-Dawley (SD) rats were randomized into control group, AP group and CQCQD group (n = 3 x 10). The rats in the CQCQD group were intragastrically administered with CQCQD (10 mL/kg every 2 h) after induction of AP by intraperitoneal injection of caerulein (50 microg/kg.h x 5) within 4 h. At 6 h after the induction of AP model, pancreatic tissues were collected for the pathological observation, mRNA extraction for determination of SERCA1 and SERCA2 mRNA expression or pancreatic acinar cell isolation for measurement of fluorescence intensity (FI) of intracellular calcium ion concentration [Ca2+]i. There was no expression of pancreatic SERCA1 mRNA in the control group and the AP group. The expression of pancreatic SERCA2 mRNA in the AP group was down-regulated (expression ratio = 0.536; P = 0.001) compared with the control group, while that in the CQCQD group was up-regulated (expression ratio = 2.00; P = 0.012) compared with AP group. The FI of intracellular [Ca2+] of pancreatic acinar cells in the AP group (138.2 +/- 23.1) was higher than...Continue Reading

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Citations

Sep 19, 2009·World Journal of Gastroenterology : WJG·Li-Hui DengQing Xia
Jun 13, 2012·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Jiaping HuaiZhiming Huang
Nov 14, 2012·Asian Pacific Journal of Tropical Medicine·Wei WuPing Xue
Mar 16, 2017·Phytotherapy Research : PTR·Pratibha AnchiChandraiah Godugu
Mar 11, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Mi Na YoonHyung Seo Park
Aug 30, 2017·Molecular Medicine Reports·Shishuai MengWeihui Zhang

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