Effect of change in an inhibitory neurotransmitter of the myenteric plexus on the pathogenetic mechanism of irritable bowel syndrome subgroups in rat models
Abstract
In order to investigate the effect of change in an inhibitory neurotransmitter of the myenteric plexus on irritable bowel syndrome (IBS) subgroups, the amounts of nitric oxide (NO) in constipation-predominant (C-IBS) and diarrhea-predominant (D-IBS) IBS models in rats were studied. The D-IBS model was created in rats by intracolonic instillation of acetic acid and by restraint stress. The D-IBS control group underwent intracolonic instillation with saline instead. The C-IBS model was created in rats by gastric instillation of 0-4 degrees C cool water daily for 14 days. The C-IBS control group underwent gastric instillation with saline instead. A blank control group was also made. Viscerosomatic sensitivity was assessed with electromyographic (EMG). Abdominal contractions induced by distension of a colonically inserted balloon (0-1.6 mL) was recorded in rats by implanting electrodes in the abdominal external oblique muscle. An India ink gastric instillation experiment was used to detect the bowel movement and fecal pellets formation. Histological analysis of colonic tissue was performed. Nicotinamide dinucleotide phosphate (NADPH)-diaphorase staining was used to detect positive NO neurons in the myenteric plexus. When the balloo...Continue Reading
References
Irritable bowel syndrome patients show enhanced modulation of visceral perception by auditory stress
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