Effect of changes in respiratory blood parameters on equine red blood cell K-Cl cotransporter

The American Journal of Physiology
P F SpeakeJ S Gibson

Abstract

K influx into equine red blood cells (RBCs) was measured using 86Rb as a tracer for K under conditions designed to mimic the changes in respiratory blood parameters that occur in vivo during strenuous exercise. The effects on K influx of physiological changes in pH, cell volume, O2 tension (PO2), CO2 tension (PCO2), and bicarbonate and lactate concentrations were defined. Physiological PO2 exerted a dominant controlling influence on the H(+)-stimulated Cl-dependent K influx, consistent with effects on the K-Cl cotransporter, PO2 required for half-maximal activity was 37 +/- 3 mmHg (4.9 kPa). Although RBCs were swollen at low pH, results showed explicitly that the volume change per se had little effect on K influx. Lactate had no effect on volume- or H(+)-stimulated K influxes, nor did bicarbonate or PCO2 affect the magnitude of K influxes after these stimuli or after treatment with protein kinase/phosphatase inhibitors. These results represent the first detailed report of O2 dependence of H(+)-stimulated K-Cl cotransport in RBCs from any mammalian species. They emphasize the importance of PO2 in control of RBC K-Cl cotransport.

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Citations

Jul 6, 2000·The Journal of Physiology·P B Dunham
Sep 26, 2000·American Journal of Physiology. Cell Physiology·M C MuzyambaJ S Gibson
Feb 16, 2005·The Journal of Membrane Biology·N C AdragnaP K Lauf
Jul 23, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yuliya KucherenkoJohn S Gibson
Nov 6, 2008·Acta Physiologica·A BogdanovaM Nikinmaa
Apr 17, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Roy E WeberPhilip S Low
Mar 22, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J S GibsonJ C Ellory
Mar 18, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Morris C MuzyambaJohn S Gibson
Nov 5, 2005·The Journal of Physiology·Modest VengustGeorge Heigenhauser
Jun 10, 2006·The Journal of Physiology·Michael BerenbrinkMikko Nikinmaa
Aug 29, 2019·Frontiers in Physiology·Halima Al BalushiJohn Stanley Gibson
Mar 2, 1999·The Journal of Physiology·E H CampbellJ S Gibson
Jan 9, 2013·The Journal of Physiology·C MilliganJ S Gibson
Oct 9, 2002·Blood Cells, Molecules & Diseases·J S Gibson, J C Ellory
Aug 6, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Mikko Nikinmaa
Mar 25, 2004·Bioelectrochemistry·Asif I KhanJohn S Gibson
Mar 25, 2004·Bioelectrochemistry·Clare DrewJohn S Gibson
Apr 17, 2007·Blood Cells, Molecules & Diseases·J C ElloryJ S Gibson

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