Effect of chaperonin encoded by gene 146 on thermal aggregation of lytic proteins of bacteriophage EL Pseudomonas aeruginosa

Biochemistry. Biokhimii︠a︡
Pavel I SemenyukL P Kurochkina

Abstract

Investigation of the chaperonin encoded by gene 146 of bacteriophage EL Pseudomonas aeruginosa that we characterized earlier has been continued. To reveal the mechanism of its functioning, new recombinant substrate proteins, fragments of gene product (gp) 183 containing the lysozyme domain were prepared. Their interaction with gp146 was studied. The influence of the phage chaperonin on the thermal aggregation of one of these gp183 fragments and endolysin (gp188) was investigated in both the presence and the absence of ATP by dynamic light scattering. In the absence of ATP, the phage chaperonin forms stable complexes with substrate proteins, thereby protecting them against thermal aggregation. Experimental data obtained for different substrate proteins are analyzed.

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Citations

Apr 28, 2020·PloS One·Andreas BracherManajit Hayer-Hartl
May 15, 2018·Frontiers in Molecular Biosciences·Jay M BhattRicardo A Bernal
Aug 9, 2020·Frontiers in Molecular Biosciences·Alejandro RodriguezRicardo A Bernal

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