Effect of chemical adjuvants on DNA vaccination

Vaccine
Huali JinBin Wang

Abstract

DNA vaccination is useful for generating immune responses, particularly the cell-mediated immune response, in a wide variety of species. However, DNA vaccination generally induces only relatively weak responses; hence, various approaches have been developed recently in order to improve its efficacy or immunopotency. The use of a chemical adjuvant is one of them. Previously we have shown that Bupivacaine or Marcaine can modulate immune responses induced by DNA vaccines [Proc. Natl. Acad. Sci. 90 (1993) 4156]. Following that lead, we have recently tested several additional chemicals for their usefulness as adjuvants in DNA inoculation. Of a total of five chemicals tested, levamisole exhibited strongest Th1 stimulatory activity whereas Tween 80 showed weakest Th1 activity, as determined by IgG2a production, and saline formulation induced weak T cell proliferation and DTH, in animals inoculated with a DNA construct expressing the foot-mouth disease viral capsule protein VP1. Furthermore, co-inoculation of levamisole increased the production of IFN-gamma by more than 100-fold as compared to that by DNA inoculation formulated in saline. In contrast, a previously reported chemical adjuvant, bupivacaine, stimulated only modest levels o...Continue Reading

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Citations

May 5, 2005·DNA and Cell Biology·Ailiang ZhangBin Wang
Apr 6, 2012·Biological Trace Element Research·Mohammad Hossein YazdiAhmad Reza Shahverdi
Sep 22, 2006·Viral Immunology·Jiangmei YinBin Wang
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Jan 6, 2007·Biochemical and Biophysical Research Communications·Youmin KangBin Wang
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Jun 17, 2014·Microbiology and Immunology·Yuewei ZhangWenxue Wu
Feb 15, 2005·Biochemical and Biophysical Research Communications·Huali JinBin Wang
Aug 13, 2005·Vaccine·Youmin KangBin Wang
Jun 29, 2005·Journal of Biotechnology·Kaichun ZhuBin Wang
Jan 10, 2012·Immunological Investigations·Youmin KangBin Wang

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