Effect of chemical and physical factors to improve the germination rate of Echinacea angustifolia seeds

Colloids and Surfaces. B, Biointerfaces
Duan ChuanrenZhao Huan

Abstract

Seeds of Echinacea angustifolia are known for their deep dormancy. In this paper, we studied the responses of E. angustifolia seeds to some chemical and physical factors, such as scarification, chilling (5 degrees C) period, light and applied BA (6-benzylaminopurine), GA3 (gibberellic acid) and sound stimulation. When the seed coat layers were removed, the germination rate grew up from 6 to 20% (incubated in light) and the mean time germination (MTG) was reduced from 18 to 6.6 days. On the basis of layers-removed, chilling and continuous light gave significantly higher germination rate (up to 70%). Compare the data of seeds chilled by 0, 6, 12, 18 and 24 days, the maximum germination rate (up to 70%) achieved at 18-days chilling treatment. Further increases in the chilling period could slightly improve germination. Exogenous application of 0.1, 0.2, 0.3 mg/L GA3 or BA in the previous pretreatment increased germination to 78, 90 and 84% or 76, 86 and 84%, respectively. Obviously, the best concentration of GA or BA is 0.3 mg/L. And the GA3 or BA treatment shortened the MTG to about 4 days. The influence of sound stimulation was also tested in the experiment. The result showed that one 100 dB and 1000 Hz sound wave (sine-wave) was...Continue Reading

References

Aug 1, 1991·Planta medica·B H SchulthessT W Baumann
Feb 13, 2001·Colloids and Surfaces. B, Biointerfaces·W BochuA Sakanishi
Jul 1, 1997·The Plant Cell·J. D. Bewley

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Citations

May 7, 2005·Colloids and Surfaces. B, Biointerfaces·Hong Bo ShaoQun Sun
Jun 28, 2005·Colloids and Surfaces. B, Biointerfaces·Zhu LiqingDuan Chuanren
Jun 28, 2016·Journal of Experimental Botany·Ratnesh Chandra MishraHanhong Bae
Oct 14, 2020·Plant Signaling & Behavior·Francesca FrongiaLaura Arru

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