PMID: 19912872Aug 1, 1992Paper

Effect of chemical depolarization on membrane recycling in hypothalamic neurons in culture

Molecular and Cellular Neurosciences
Y KagotaniA Tixier-Vidal

Abstract

The mechanisms of membrane recycling and endocytic activity have been investigated by morphological methods, following chemical depolarization, in cultured mouse hypothalamic neurons that have developed in vitro fully differentiated synapses. Previous studies with this cell system have shown that short-term (3 min) exposure to a depolarizing concentration of KCl induces a depletion of synaptic vesicles in synaptic boutons. Subsequent return to control medium is followed by a restoration of synaptic vesicle numbers. We have now used horseradish peroxidase (HRP) as a tracer for endocytosis and wheat-germ agglutinin coupled to HRP-gold as a marker for adsorptive endocytosis. In parallel, synaptophysin, a marker for small synaptic vesicles, was simultaneously localized by electron microscopic immunocytochemistry in some experiments. The morphometric analyses and the time course of HRP uptake combined with the distribution of synaptophysin immunostaining indicate that short-term (3 min) depolarization leads to a depletion of synaptic vesicles together with an enhanced endocytic activity via vesicles that possess both the diameter and the major membrane protein, synaptophysin, characteristic of synaptic vesicles. These findings sugge...Continue Reading

References

Dec 1, 1978·The Journal of Cell Biology·S F Schaeffer, E Raviola
Jan 1, 1990·Annual Review of Physiology·P De Camilli, R Jahn
Aug 1, 1988·Neuron·R B Kelly
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R JahnP Greengard
Jan 1, 1983·The Journal of Cell Biology·R M SteinmanZ A Cohn
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