Abstract
Upon ingestion of particulate matter, polymorphonuclear leukocytes produce a chemiluminescence that can be measured in a liquid scintillation counter. In the experiments reported here, the influence of three chemoattractants and three chemotactic modulators upon the chemiluminescence induced by opsonized zymosan was studied. The chemoattractants investigated (including bacterial factor derived from Escherichia coli, the simple peptide formylmethionylalanine, and activated human complement), which initiate directed movement when presented to cells in a concentration gradient, significantly enhanced zymosan-induced chemiluminescence. In the absence of opsonized zymosan, however, they had no effect on the chemiluminescence response. In contrast, the chemotactic modulators studied (including carbamylcholine, phenylephrine, and cyclic guanosine 5'-monophosphate, which are not chemotactic by themselves but can enhance or depress the movement of polymorphonuclear leukocytes initiated by chemoattractants) produced no enhancement of chemiluminescence. Other experiments were carried out in which neutrophils were pretreated with cytochalasin D, a compound that inhibits phagocytosis by interacting with microfilaments. Under these condition...Continue Reading
References
Dec 1, 1976·The Journal of Clinical Investigation·V G HemmingH R Hill
Mar 1, 1975·Metabolism: Clinical and Experimental·H R HillN D Goldberg
Jun 1, 1975·The Journal of Clinical Investigation·R B JohnstonK V RaJagopalan
Jun 1, 1976·The Journal of Experimental Medicine·R B JohnstonL A Guthrie
Feb 1, 1977·The Journal of Clinical Investigation·I M GoldsteinH B Kaplan
Jan 1, 1977·International Archives of Allergy and Applied Immunology·H R HillV G Hemming
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E SchiffmannS M Wahl
May 27, 1976·Nature·N Mimura, A Asano
Oct 8, 1974·Biochemical and Biophysical Research Communications·R C AllenR H Steele
May 26, 1972·Biochemical and Biophysical Research Communications·R C AllenR H Steele
Citations
Jan 1, 1987·Acta Anaesthesiologica Scandinavica·J PerttiläA Rajamäki
Jan 1, 1981·Critical Reviews in Clinical Laboratory Sciences·J SchadelinG L Mandell
Jan 1, 1994·Journal of Biomaterials Science. Polymer Edition·G NimeriH Elwing
Feb 1, 1989·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·O Paulsen, A Forsgren
Aug 1, 1986·Immunological Investigations·W D BiggarG Kent
Jan 1, 1988·Microbiology and Immunology·Y FukaseF Sendo
Dec 1, 1985·European Journal of Clinical Microbiology·A Forsgren, P I Bergkvist
Jul 1, 1988·Journal of Clinical Periodontology·B Asman
Jan 1, 1990·Cancer Immunology, Immunotherapy : CII·F KonishiK Nomoto
Feb 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·A Scheja, A Forsgren
Apr 1, 1993·Scandinavian Journal of Clinical and Laboratory Investigation·K Bergström, B Asman
Feb 19, 1998·Oral Diseases·A GustafssonK Bergström
Jan 1, 1996·Comparative Immunology, Microbiology and Infectious Diseases·R Cooray
May 1, 1979·Infection and Immunity·A C IssekutzW D Biggar
Feb 1, 1981·Journal of Clinical Microbiology·E Prendergast, R A Proctor
Aug 13, 1980·Biochimica Et Biophysica Acta·C D AllredH R Hill
Sep 1, 1983·Infection and Immunity·J G Bender, D E Van Epps
Aug 1, 1980·Infection and Immunity·S LöfgrenJ Carlsson
Jul 1, 1982·Infection and Immunity·C Dahlgren, O Stendahl
Jan 1, 1979·Infection and Immunity·J W SixbeyC M Nolan
Dec 1, 1980·Infection and Immunity·J P McCarthyP Z Sobocinski
Feb 1, 1983·Infection and Immunity·C Dahlgren, O Stendahl
Dec 10, 1989·Transfusion Science·W van OeverenM D Kazatchkine
Sep 28, 1984·Biochemical and Biophysical Research Communications·P RoschgerH Klima