Effect of chilling bovine oocytes on their developmental competence

Molecular Reproduction and Development
A MartinoS P Leibo

Abstract

Bovine oocytes are damaged when chilled to temperatures near 0 degree C. We have determined the temperatures at which this injury occurs, as well as its kinetics and the functional consequences for oocytes both at the germinal vesicle-stage (GV) and after in vitro maturation (IVM). Cooling GV oocytes had no effect on their nuclear maturation or fertilization. Compared to control oocytes held at 30 degrees C, the development of GV oocytes into blastocysts following maturation and fertilization was unaffected by cooling them to 20 degrees C for 30 min (blastocyst formation: 25% vs 26%, respectively), but development decreased after cooling them to 10 degrees C and 0 degree C (blastocyst: 6% and 1%, respectively). Cooling oocytes after maturation gave similar results, with no difference between controls and oocytes cooled to 20 degrees C (blastocyst: 25% and 26%, respectively). However, cooling them to 10 degrees C and 0 degree C did reduce development (blastocyst: 8% and 3%, respectively). Chilling oocytes to 0 degree C for 30 sec reduced their cleavage and blastocyst formation by > 40%; there was a high negative correlation between the length of exposure and subsequent survival, both for GV-stage and for IVM oocytes. The extreme...Continue Reading

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Citations

Feb 24, 2006·Journal of Assisted Reproduction and Genetics·S Balasubramanian, Gyu-Jin Rho
Jul 16, 2009·Journal of Assisted Reproduction and Genetics·Teraporn VutyavanichSiriporn Nunta
Apr 17, 2014·BioMed Research International·In-Sul Hwang, Shinichi Hochi
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Mar 25, 2015·Zygote : the Biology of Gametes and Early Embryos·Qian Li, Long-Bo Cui
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Jul 6, 2004·Molecular Reproduction and Development·Gyu-Jin RhoSang-Young Choe
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Feb 15, 2001·Molecular Reproduction and Development·M Lane, D K Gardner
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