Effect of chitosan-alginate nanoparticles and ultrasound on the efficiency of gene transfection of human cancer cells

The Journal of Gene Medicine
Shu-Jyuan YangMing-Jium Shieh

Abstract

Gene therapy has been used to treat a variety of health problems, but transfection inefficiency and the lack of safe vectors have limited clinical progress. Fabrication of a vector that is safe and has high transfection efficiency is crucial for the development of successful gene therapy. The present study aimed to synthesize chitosan-alginate nanoparticles that can be used as carriers of the pAcGFP1-C1 plasmid and to use these nanoparticles with an ultrasound protocol to achieve high efficiency gene transfection. Chitosan was complexed with alginate and the pAcGFP1-C1 plasmid at different charge ratios to create chitosan-alginate-DNA nanoparticles (CADNs). The average particle size and loading efficiency were measured. Plasmid DNA retardation and integrity were analysed on 1% agarose gels. The effect of CADNs and ultrasound on the efficiency of transfection of cells and subcutaneous tumors was evaluated. In the CADNs, the average size of incorporated plasmid DNA was 600-650 nm and the loading efficiency was greater than 90%. On the basis of the results of the plasmid DNA protection test, CADNs could protect the transgene from DNase I degradation. The transgene product expression could be enhanced efficiently if cells or tumor ...Continue Reading

References

Mar 1, 1990·Trends in Biotechnology·O Smidsrød, G Skjåk-Braek
Nov 9, 1992·Biochimica Et Biophysica Acta·H FarhoodL Huang
Oct 1, 1989·The Journal of Pharmacy and Pharmacology·O N Singh, J Burgess
Oct 2, 1989·Biochimica Et Biophysica Acta·P PinnaduwageL Huang
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P L FelgnerM Danielsen
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Y Legendre, F C Szoka
May 1, 1996·Journal of Microencapsulation·P R HariC P Sharma
Aug 1, 1998·Journal of Controlled Release : Official Journal of the Controlled Release Society·K Y LeeS Y Jeong
Aug 28, 1998·Human Gene Therapy·V L Truong-LeK W Leong
Mar 8, 2000·Critical Reviews in Therapeutic Drug Carrier Systems·M C Garnett
Feb 22, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·H Q MaoK W Leong
Nov 6, 2001·Critical Reviews in Eukaryotic Gene Expression·T P RichardsonD J Mooney
Nov 24, 2001·Advanced Drug Delivery Reviews·G Borchard
Mar 13, 2002·Circulation·Yoshiaki TaniyamaRyuichi Morishita
Apr 30, 2002·Ultrasound in Medicine & Biology·Shao-Ling HuangRobert C MacDonald
Apr 4, 2003·International Journal of Pharmaceutics·Shuying GaoJunfeng Zhang
Jan 20, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Sania MansouriJulio C Fernandes
May 4, 2004·Ultrasound in Medicine & Biology·Vladimir G Zarnitsyn, Mark R Prausnitz
Oct 9, 2004·Biochimica Et Biophysica Acta·Shao-Ling Huang, Robert C MacDonald
Oct 20, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sunil A AgnihotriTejraj M Aminabhavi
Mar 19, 2005·Technology in Cancer Research & Treatment·Irina V LarinaRinat O Esenaliev
Nov 22, 2005·Expert Opinion on Drug Delivery·William G PittBryant J Staples
Jan 25, 2006·Investigative Ophthalmology & Visual Science·Shozo SonodaTaiji Sakamoto
Jun 24, 2006·Cancer·Alex G CuencaStephen R Grobmyer
Oct 19, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kimberly L DouglasMaryam Tabrizian
Nov 23, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ryo SuzukiKazuo Maruyama
Feb 16, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Azadeh KheirolomoomKatherine W Ferrara
Mar 7, 2007·Gene Therapy·C M H Newman, T Bettinger
May 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sergio DromiBradford J Wood
Jun 15, 2007·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·H Y FangW S Chen
Jul 30, 2009·The Journal of Gene Medicine·Kun-Che TsaiWen-Shiang Chen

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Citations

May 28, 2010·Pharmaceutical Research·Michiro SusaZhenfeng Duan
Nov 25, 2011·International Journal of Nanomedicine·Ziyad Mohammed BinsalamahDominique Shum-Tim
Jul 31, 2012·Therapeutic Delivery·Hunghao Chu, Yadong Wang
Mar 22, 2016·Expert Opinion on Biological Therapy·Ahad MokhtarzadehMohammad Ramezani
Jul 27, 2014·International Journal of Pharmaceutics·Diana Guzman-VillanuevaHugh D C Smyth
Feb 12, 2014·Acta Biomaterialia·Jyothi U MenonKytai T Nguyen
Dec 5, 2013·Carbohydrate Polymers·Wei-Wen Hu, Shiang-Lung Tsou
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Maryam ZohriMohammad Hossein Ghahremani

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