Effect of cholesterol content on affinity and stability of factor VIII and annexin V binding to a liposomal bilayer membrane

Chemistry and Physics of Lipids
Jun Yeoung JeonEun Kyu Lee

Abstract

To investigate the effect of cholesterol composition on the binding of factor VIII (FVIII) and annexin V (AV) to membranes, liposomal membranes with phospholipid bilayers of various compositions of phosphatidylcholine (PC), phosphatidylserine (PS), and cholesterol were constructed. A surface plasmon resonance (SPR) biosensor system was employed to measure the equilibrium and rate constants of the bindings. As expected, PS was found to play a dominant role in the binding of AV; its binding level was directly proportional to the PS composition in a liposome. The binding levels of FVIII and AV to liposome increased with an increase in cholesterol composition in liposome. It seemed to suggest that cholesterol in liposome acts as a 'phospholipid arrangement' factor by inducing the formation of PS-rich microdomains. However, in the absence of PS (20% on a mole basis), cholesterol could not exert the binding enhancement effect, which again confirmed the critical role of PS in the bindings. Stability of the AV binding was significantly improved by the increase in cholesterol content; for AV, the dissociation rate constant was decreased approximately fivefold, from 1.7 x 10(-3)s(-1) in the absence of cholesterol to 3.3 x 10(-4)s(-1) in ...Continue Reading

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Citations

Nov 28, 2012·Analytical and Bioanalytical Chemistry·Y OlguínR Galindo
Aug 11, 2011·Apoptosis : an International Journal on Programmed Cell Death·Sang Youn HwangE K Lee
Dec 14, 2011·Cellular and Molecular Life Sciences : CMLS·Magdalena DomonJoanna Bandorowicz-Pikula
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Sep 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ngonidzashe Ruwizhi, Blessing Atim Aderibigbe

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