Abstract
To investigate the effect of cholesterol composition on the binding of factor VIII (FVIII) and annexin V (AV) to membranes, liposomal membranes with phospholipid bilayers of various compositions of phosphatidylcholine (PC), phosphatidylserine (PS), and cholesterol were constructed. A surface plasmon resonance (SPR) biosensor system was employed to measure the equilibrium and rate constants of the bindings. As expected, PS was found to play a dominant role in the binding of AV; its binding level was directly proportional to the PS composition in a liposome. The binding levels of FVIII and AV to liposome increased with an increase in cholesterol composition in liposome. It seemed to suggest that cholesterol in liposome acts as a 'phospholipid arrangement' factor by inducing the formation of PS-rich microdomains. However, in the absence of PS (20% on a mole basis), cholesterol could not exert the binding enhancement effect, which again confirmed the critical role of PS in the bindings. Stability of the AV binding was significantly improved by the increase in cholesterol content; for AV, the dissociation rate constant was decreased approximately fivefold, from 1.7 x 10(-3)s(-1) in the absence of cholesterol to 3.3 x 10(-4)s(-1) in ...Continue Reading
References
Mar 1, 1992·Calcified Tissue International·D Skrtic, E D Eanes
Jun 30, 1987·Biochemical and Biophysical Research Communications·J Sánchez-YagüeM Llanillo
Dec 7, 1983·Biochimica Et Biophysica Acta·E M BeversR F Zwaal
Mar 2, 1995·Biochimica Et Biophysica Acta·J W HeemskerkG Hornstra
May 25, 1993·Biochemistry·P Meers, T Mealy
Dec 15, 1996·Journal of Cellular Biochemistry·B RamjiawanG N Pierce
Mar 1, 1997·Molecular Biology of the Cell·T HarderJ Gruenberg
Jun 1, 1997·Cellular and Molecular Life Sciences : CMLS·C P Reutelingsperger, W L van Heerde
Feb 5, 1998·Cytometry·M van EngelandC P Reutelingsperger
Dec 23, 1998·Biochemistry·D HusterK Gawrisch
Apr 7, 1999·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·B A Seaton, J R Dedman
Sep 10, 1999·Journal of Chromatography. a·E SaenkoD Josic
May 9, 2001·Biochimica Et Biophysica Acta·J Ayala-SanmartinL A Pradel
Jul 20, 2001·Journal of Chromatography. a·E SaenkoD Josić
Dec 26, 2001·Chemical Reviews·A C Newton
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Oct 1, 2005·Journal of Thrombosis and Haemostasis : JTH·G L Dale
Mar 8, 2006·Biochemistry·Monika Damek-PoprawaKathleen Boesze-Battaglia
Jan 9, 2007·Biochimica Et Biophysica Acta·Stefania ReineriFabiola Sinigaglia
Mar 14, 2007·Free Radical Biology & Medicine·Abel López-RevueltaMarcial Llanillo
Aug 1, 2009·Journal of Fluorescence·Tanja MeierhoferA Toby A Jenkins
Citations
Nov 28, 2012·Analytical and Bioanalytical Chemistry·Y OlguínR Galindo
Aug 11, 2011·Apoptosis : an International Journal on Programmed Cell Death·Sang Youn HwangE K Lee
Dec 14, 2011·Cellular and Molecular Life Sciences : CMLS·Magdalena DomonJoanna Bandorowicz-Pikula
Mar 9, 2012·Colloids and Surfaces. B, Biointerfaces·Sang Youn HwangE K Lee
Nov 9, 2010·Advances in Pediatrics·Marilyn J Manco-Johnson
Nov 4, 2015·Colloids and Surfaces. B, Biointerfaces·Jae Sun LeeE K Lee
Nov 25, 2014·The Journal of Antimicrobial Chemotherapy·Venkata Saran SolletiAbdelwahab Omri
Jul 18, 2016·Colloids and Surfaces. B, Biointerfaces·Sujin JungE K Lee
Sep 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ngonidzashe Ruwizhi, Blessing Atim Aderibigbe