PMID: 8448163Mar 2, 1993Paper

Effect of cholesterol oxidase treatment on physical state of renal brush border membranes: evidence for a cholesterol pool interacting weakly with membrane lipids

Biochemistry
E H el Yandouzi, C Le Grimellec

Abstract

Treatment with cholesterol oxidases has shown that cholesterol is heterogeneously distributed in brush borders isolated from the apical membrane domain of the renal and intestinal epithelial cells [Bloj, B., & Zilversmit, D. B. (1982) J. Biol. Chem. 257, 7608-7614; El Yandouzi, E. H., & Le Grimellec, C. (1992) Biochemistry 31, 547-551]. To better understand the origin of cholesterol heterogeneity, the effects of cholesterol oxidation by Brevibacterium sp. cholesterol oxidase on the physical state of renal brush border membrane vesicles were determined using steady-state fluorescence polarization and differential phase fluorescence of 1,6-diphenyl-1,3,5-hexatriene (DPH). Selective quenching by trinitrobenzenesulfonate indicated that DPH distributes equally between outer and inner membrane leaflets. Oxidation of 90% of the cholesterol decreased the steady-state anisotropy of DPH, determined at 37 degrees C, by 14%. This modification corresponded to a change in the lipid order, the rotational rate of the probe being unaffected. Oxidation of the cholesterol corresponding to the readily accessible pool (30% of the total cholesterol), on the other hand, had a very limited effect on the membrane physical state. This contrasted with th...Continue Reading

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Citations

Sep 6, 1994·Chemistry and Physics of Lipids·R Welti, M Glaser
Mar 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·F BellemareC Malo
Oct 22, 2009·The FEBS Journal·Joseph Kreit, Nicole S Sampson
Apr 18, 2006·Progress in Lipid Research·Thomas J Pucadyil, Amitabha Chattopadhyay
Apr 27, 2005·Biochemical and Biophysical Research Communications·Thomas J PucadyilAmitabha Chattopadhyay
Oct 4, 2005·Biophysical Journal·Thomas PlénatChristian Le Grimellec
Jun 26, 2001·Biophysical Journal·P E MilhietC Le Grimellec
Oct 3, 2009·Biochimica Et Biophysica Acta·Marie-Cécile GiocondiChristian Le Grimellec
Jan 23, 2017·Chemistry and Physics of Lipids·Md JafurullaAmitabha Chattopadhyay
Oct 1, 2005·The Journal of Biological Chemistry·Robert F Jacob, R Preston Mason
Nov 22, 2001·The Journal of Biological Chemistry·Pierre Emmanuel MilhietChristian Le Grimellec

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