PMID: 9437743Jan 23, 1998Paper

Effect of chronic administration of [D-Pen2, D-Pen5] enkephalin on the activity of nitric oxide synthase in brain regions and spinal cord of mice

Peptides
H N Bhargava, Y J Cao

Abstract

The effect of multiple intracerebroventricular (i.c.v.) injections of [D-Pen2, D-Pen5]enkephalin (DPDPE), a delta-opioid receptor agonist, on the activity of nitric oxide synthase (NOS) was determined in the brain regions and spinal cord of the mouse. Male Swiss Webster mice were injected twice daily with DPDPE (20 micrograms/mouse, i.c.v.) or its vehicle for 4 days. This procedure has previously been shown to induce tolerance to the antinociceptive actions of DPDPE in mice. On day 5, the animals treated with DPDPE were either sacrificed 20 min after an i.c.v. injection of DPDPE (tolerant) or without any injection (abstinent i.e., 16 h after the last injection of DPDPE). NOS activity in brain regions (cortex, striatum, hippocampus, midbrain, pons/medulla, hypothalamus and cerebellum) and spinal cord was determined by the rate of conversion of arginine into citrulline. Tolerance to DPDPE was associated increases in NOS activity in midbrain (49%) and pons/medulla (32%) and decreases in cerebellum (28%) and spinal cord (44%). However, NOS activity was unchanged in the cortex, corpus striatum, hippocampus and hypothalamus. On the other hand, during abstinence from DPDPE, NOS activity increased in midbrain (84%) and hypothalamus (35...Continue Reading

References

Jan 1, 1992·Neuron·D S Bredt, S H Snyder
Feb 1, 1991·Trends in Neurosciences·J Garthwaite
Aug 1, 1991·European Journal of Clinical Investigation·S Moncada, E A Higgs
Dec 16, 1991·Biochemical and Biophysical Research Communications·A CalignanoM Di Rosa
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R G KnowlesS Moncada
Sep 23, 1994·Cell·C Nathan, Q W Xie
Nov 1, 1994·Neuropharmacology·A M BabeyG W Pasternak
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y A KolesnikovG W Pasternak
Sep 3, 1993·Brain Research·S N ThoratH N Bhargava
Jan 1, 1995·NIDA Research Monograph·G W Pasternak
Oct 4, 1995·European Journal of Pharmacology·J C LezaP Lorenzo
Mar 1, 1957·British Journal of Pharmacology and Chemotherapy·T J HALEY, W G MCCORMICK

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.