Abstract
To determine the effect of cis -9, trans -11-conjugated linoleic acid (c9, t11-CLA) on the cell cycle of gastric cancer cells (SGC-7901) and its possible mechanism in inhibition cancer growth. Using cell culture and immunocytochemical techniques, we examined the cell growth, DNA synthesis, expression of PCNA, cyclin A, B(1), D(1), p16(ink4a) and p21(cip/waf1) of SGC-7901 cells which were treated with various c9, t11-CLA concentrations (25, 50, 100 and 200 micromol.L(-1))of c 9, t 11-CLA for 24 and 48h, with a negative control (0.1% ethane). The cell growth and DNA synthesis of SGC-7901 cells were inhibited by c9, t11-CLA.SGC-7901 cells. Eight day after treatment with various concentrations of c9, t11-CLA mentioned above, the inhibition rates were 5.92%, 20.15%, 75.61% and 82.44%, respectively and inhibitory effect of c9, t11-CLA on DNA synthesis (except for 25 micromol.L, 24h) showed significantly less (3)H-TdR incorporation than that in the negative controls (P<0.05 and P<0.01). Immunocytochemical staining demonstrated that SGC-7901 cells preincubated in media supplemented with different c9, t11-CLA concentrations at various times significantly decreased the expressions of PCNA (the expression rates were 7.2-3.0%, 24h and 9.1-...Continue Reading
References
Mar 20, 1999·The Journal of Nutrition·A E BrodieC Y Hu
Jun 8, 1999·The American Journal of Clinical Nutrition·C J Lewis, E A Yetley
Jul 1, 1999·Journal of Dairy Science·P W Parodi
Nov 26, 1999·The Journal of Nutrition·C IpD Bauman
Nov 30, 1999·Frontiers in Bioscience : a Journal and Virtual Library·T Tsurimoto
Apr 12, 2000·Journal of the American College of Nutrition·H B MacDonald
Jun 1, 2000·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·A MathewM K Nair
Jun 29, 2000·The American Journal of Clinical Nutrition·E MantziorisM J James
Oct 13, 2000·Gastroenterology Clinics of North America·K Kimura
Nov 4, 2000·Expert Opinion on Investigational Drugs·S ManiR Pestell
Nov 23, 2000·Plant Molecular Biology·M Ito
Feb 13, 2001·Current Opinion in Genetics & Development·S Pyronnet, N Sonenberg
Feb 24, 2001·Stem Cells·E D Israels, L G Israels
Feb 24, 2001·BioFactors·Y Sowa, T Sakai
May 18, 2001·Cancer radiothérapie : journal de la Société française de radiothérapie oncologique·J F ViallardJ Reiffers
May 23, 2001·Cancer Letters·N KimotoT Shirai
Aug 24, 2001·Toxicology·S SugieH Mori
Jan 31, 2002·World Journal of Gastroenterology : WJG·Lin CaiZuo-Feng Zhang
Jan 31, 2002·World Journal of Gastroenterology : WJG·Fang-Xin ZhangJun-Jie Fan
Jan 31, 2002·World Journal of Gastroenterology : WJG·Qin-Long GuYan-Zhen Lin
Jan 31, 2002·World Journal of Gastroenterology : WJG·Lin CaiYing-Nan Yi
Jan 31, 2002·World Journal of Gastroenterology : WJG·Heng-Jun GaoZhi-Quan Zhao
Jan 31, 2002·World Journal of Gastroenterology : WJG·Hong-Ping ZhouNan-Zheng Zhang
Jan 31, 2002·World Journal of Gastroenterology : WJG·K WuB H Liu
Jan 31, 2002·World Journal of Gastroenterology : WJG·K WuY Zhao
Jan 31, 2002·World Journal of Gastroenterology : WJG·W X NiuC P Wang
Jan 31, 2002·World Journal of Gastroenterology : WJG·R T CuiT Tian
Jan 31, 2002·World Journal of Gastroenterology : WJG·L CaiZ F Zhang
Jan 31, 2002·World Journal of Gastroenterology : WJG·X S HeL R Zhang
Jan 31, 2002·World Journal of Gastroenterology : WJG·D G LiH M Lu
Jan 31, 2002·World Journal of Gastroenterology : WJG·S LiuW J Su
Citations
Nov 20, 2002·World Journal of Gastroenterology : WJG·Kun WuLan Zhao
Jan 1, 2003·World Journal of Gastroenterology : WJG·Bing-Qing ChenRui-Hai Liu
Jul 11, 2003·World Journal of Gastroenterology : WJG·Ya-Fei MaoShu-Ping Li
Sep 13, 2003·World Journal of Gastroenterology : WJG·Bing-Qing ChenRui-Hai Liu
Jan 13, 2004·World Journal of Gastroenterology : WJG·Jia-Ren LiuYou-Qiang Song
Feb 5, 2004·World Journal of Gastroenterology : WJG·Jia-Ren LiuYou-Qiang Song
Mar 31, 2004·World Journal of Gastroenterology : WJG·Jie Yan, Ya-Fei Mao
Mar 12, 2020·Frontiers in Endocrinology·Arpna SharmaJens Vanselow
May 11, 2004·World Journal of Gastroenterology : WJG·Bing-Qing ChenRui-Hai Liu
Feb 15, 2012·Advances in Nutrition·Sarah K GebauerDavid J Baer
Nov 20, 2002·World Journal of Gastroenterology : WJG·Jia-Ren LiuRui-Hai Liu
Sep 13, 2003·World Journal of Gastroenterology : WJG·Xiao-Feng YeWen-Jin Su
Dec 21, 2004·World Journal of Gastroenterology : WJG·Hong-Bin CuiYing Liu
Jan 1, 2010·Nutrition and Cancer·Jia-Ren LiuBao-Feng Yang
Mar 31, 2004·Canadian Journal of Physiology and Pharmacology·Geneviève DrouinBenoit Paquette
Nov 20, 2002·World Journal of Gastroenterology : WJG·Jian-Kun HuYou-Ping Li
Oct 17, 2003·World Journal of Gastroenterology : WJG·Jie YanShu-Ping Li
Apr 8, 2004·World Journal of Gastroenterology : WJG·Jie YanYi-Hui Luo
Mar 25, 2004·World Journal of Gastroenterology : WJG·Jie YanYa-Fei Mao
Mar 31, 2004·World Journal of Gastroenterology : WJG·Ya-Fei Mao, Jie Yan
Jan 28, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Lu WangJia-Ren Liu
Oct 12, 2005·Biochimica Et Biophysica Acta·Kimberly M HargraveJess L Miner
Apr 12, 2013·Inorganic Chemistry·Lu WangGuo-Yu Yang
Feb 5, 2011·The Journal of Nutritional Biochemistry·Yang LiJia-Ren Liu