Effect of cis-9, trans-11-conjugated linoleic acid on cell cycle of gastric adenocarcinoma cell line (SGC-7901)

World Journal of Gastroenterology : WJG
Jia-Ren LiuRui-Hai Liu

Abstract

To determine the effect of cis -9, trans -11-conjugated linoleic acid (c9, t11-CLA) on the cell cycle of gastric cancer cells (SGC-7901) and its possible mechanism in inhibition cancer growth. Using cell culture and immunocytochemical techniques, we examined the cell growth, DNA synthesis, expression of PCNA, cyclin A, B(1), D(1), p16(ink4a) and p21(cip/waf1) of SGC-7901 cells which were treated with various c9, t11-CLA concentrations (25, 50, 100 and 200 micromol.L(-1))of c 9, t 11-CLA for 24 and 48h, with a negative control (0.1% ethane). The cell growth and DNA synthesis of SGC-7901 cells were inhibited by c9, t11-CLA.SGC-7901 cells. Eight day after treatment with various concentrations of c9, t11-CLA mentioned above, the inhibition rates were 5.92%, 20.15%, 75.61% and 82.44%, respectively and inhibitory effect of c9, t11-CLA on DNA synthesis (except for 25 micromol.L, 24h) showed significantly less (3)H-TdR incorporation than that in the negative controls (P<0.05 and P<0.01). Immunocytochemical staining demonstrated that SGC-7901 cells preincubated in media supplemented with different c9, t11-CLA concentrations at various times significantly decreased the expressions of PCNA (the expression rates were 7.2-3.0%, 24h and 9.1-...Continue Reading

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Citations

Jul 11, 2003·World Journal of Gastroenterology : WJG·Ya-Fei MaoShu-Ping Li
Sep 13, 2003·World Journal of Gastroenterology : WJG·Bing-Qing ChenRui-Hai Liu
Jan 13, 2004·World Journal of Gastroenterology : WJG·Jia-Ren LiuYou-Qiang Song
Feb 5, 2004·World Journal of Gastroenterology : WJG·Jia-Ren LiuYou-Qiang Song
Mar 12, 2020·Frontiers in Endocrinology·Arpna SharmaJens Vanselow
May 11, 2004·World Journal of Gastroenterology : WJG·Bing-Qing ChenRui-Hai Liu
Sep 13, 2003·World Journal of Gastroenterology : WJG·Xiao-Feng YeWen-Jin Su
Dec 21, 2004·World Journal of Gastroenterology : WJG·Hong-Bin CuiYing Liu
Mar 31, 2004·Canadian Journal of Physiology and Pharmacology·Geneviève DrouinBenoit Paquette
Nov 20, 2002·World Journal of Gastroenterology : WJG·Jian-Kun HuYou-Ping Li
Mar 25, 2004·World Journal of Gastroenterology : WJG·Jie YanYa-Fei Mao
Jan 28, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Lu WangJia-Ren Liu
Feb 5, 2011·The Journal of Nutritional Biochemistry·Yang LiJia-Ren Liu

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