Effect of clenbuterol on non-endothelial nitric oxide release in rat mesenteric arteries and the involvement of beta-adrenoceptors

British Journal of Pharmacology
J Marín, G Balfagón

Abstract

1. The aim of the present study was to explore the contribution of adrenergic, sensory and nitrergic innervations to the inhibitory effects of the beta2-adrenoceptor agonist clenbuterol on responses to electrical field stimulation (EFS, 200 mA, 0.3 ms, 1-16 Hz, for 30 s, at 1 min interval) in rat mesenteric artery segments without endothelium and the possible involvement of adrenergic, sensory and nitrergic innervations. 2. Clenbuterol (1 microM) reduced EFS-induced contractile responses, and this effect was reversed by the beta-antagonist propranolol (1 microM) (contraction at 16 Hz expressed as % of 75 mM K+-induced contraction was: control, 69+/-9, clenbuterol, 31+/-6, n=13, P<0.001; control, 83+/-5, clenbuterol+propranolol 70+/-7, n=11, P>0.05). 3. In arteries preincubated with [3H]-noradrenaline (NA), clenbuterol did not modify the tritium overflow evoked by EFS (200 mA, 0.3 ms, 4 Hz, for 60 s; ratio between tritium release in the second and first stimuli was: control, 0.80+/-0.05 and clenbuterol added before second stimulus, 0.91+/-0.11, n=5, P>0.05). 4. The nitric oxide (NO) synthase inhibitors NG-monomethyl-L-arginine (L-NMMA) and NG-nitro-L-arginine methyl ester (L-NAME) (10 and 100 microM), and the guanylate cyclase i...Continue Reading

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Citations

Mar 23, 2004·European Journal of Pharmacology·Mercedes FerrerGloria Balfagón
Aug 13, 2004·Journal of Veterinary Pharmacology and Therapeutics·C BelloliG Re
Sep 16, 2010·British Journal of Pharmacology·Javier Blanco-RiveroGloria Balfagón
Feb 7, 2006·Regulatory Peptides·Iván Márquez-RodasGloria Balfagón
May 7, 2005·Nitric Oxide : Biology and Chemistry·M del Carmen MartínMercedes Ferrer
Apr 21, 2004·Journal of Hypertension·Fabiano E XavierGloria Balfagón
Jul 17, 2015·British Journal of Pharmacology·D B de QueirozG Balfagón
Nov 7, 2000·Journal of Cardiovascular Pharmacology·M FerrerG Balfagón
Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·T J LeeS Sarwinski

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