Effect of clinically-related factors on in vitro blastocyst development after equine ICSI

Theriogenology
Young-Ho ChoiK Hinrichs

Abstract

Equine intracytoplasmic sperm injection (ICSI) is being used clinically for foal production, but little information is available on factors affecting the efficiency of this procedure. We examined factors that may influence blastocyst development when ICSI is performed clinically, i.e., on oocytes recovered from live mares by transvaginal ultrasound-guided follicle aspiration (TVA), using sperm from the stallion of the client's choice. In a clinical setting, there may be a delay from the time of TVA to isolation of oocytes from the aspirated fluid. In a preliminary study, oocytes from fluid held for 1.5 h at ambient temperature (26°C-33°C) yielded 32% blastocysts; however, in experiment 1, fluid held at 32 °C for 2 h after aspiration yielded 16% blastocysts versus 23% for aspirates processed immediately. Performing TVA/ICSI throughout the year would increase production from valuable mares, but efficiency during the nonbreeding season is unknown. In addition, to reduce the possibility of infection after TVA, administration of antibiotics to the mare before TVA is indicated; however, these could affect oocyte quality. In experiment 2, follicle numbers at the time of TVA were significantly higher in December to January than for the...Continue Reading

References

Jan 1, 1992·Equine Veterinary Journal·I BrückT Greve
Jan 7, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Joëlle A DesmaraisLawrence C Smith
Aug 19, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Y H ChoiK Hinrichs
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Apr 9, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Young-Ho ChoiKatrin Hinrichs

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Citations

May 14, 2016·Reproduction in Domestic Animals = Zuchthygiene·S D KharcheAks Sikarwar
Nov 5, 2016·The Veterinary Clinics of North America. Equine Practice·Elaine M Carnevale
Mar 3, 2018·Reproduction in Domestic Animals = Zuchthygiene·K RoelsA Van Soom
Apr 15, 2018·Equine Veterinary Journal·L H A Morris
Dec 3, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Daniel F SalamoneMaría Belén Rodríguez
Sep 22, 2018·Reproduction in Domestic Animals = Zuchthygiene·Katrin Hinrichs
Feb 15, 2018·Scientific Reports·Serafín Pérez-CerezalesAlfonso Gutiérrez-Adán
Oct 24, 2019·Reproduction, Fertility, and Development·Lee H Morris, Lisa J Maclellan
Nov 21, 2019·Reproduction, Fertility, and Development·María Belén RodríguezDaniel F Salamone
May 23, 2020·Animals : an Open Access Journal From MDPI·Pablo Fernández-HernándezBeatriz Macías-García
Oct 3, 2019·Reproduction, Fertility, and Development·Lino Fernando Campos-ChillonJoy L Altermatt
Apr 11, 2019·Reproduction, Fertility, and Development·Kaatje D DucheyneMarta de Ruijter-Villani
Jan 7, 2021·Animals : an Open Access Journal From MDPI·Brenda Florencia GimenoClaudia Elena Osycka-Salut
Mar 31, 2021·Journal of Equine Veterinary Science·Erin A HiseyStuart Meyers
Jul 15, 2018·Theriogenology·Raul A Gonzalez-Castro, Elaine M Carnevale

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