Effect of closed v. intermittent-flow respirometry on hypoxia tolerance in the shiner perch Cymatogaster aggregata

Journal of Fish Biology
S SnyderJ F Steffensen

Abstract

This study compares the critical oxygen saturation (O2 crit ) levels of the shiner perch Cymatogaster aggregata obtained using two different methods wherein hypoxia is induced either by the fish's respiration (closed respirometry) or by degassing oxygen with nitrogen (intermittent-flow respirometry). Fish exhibited loss of equilibrium at a higher O2 saturation in the closed respirometry method when compared with the intermittent-flow method. Utilization of closed respirometry yielded O2 crit measurements that were almost twice as high as those obtained with intermittent-flow respirometry. The lower hypoxia tolerance in closed respirometry is consistent with additional stress, caused by a build-up of ammonia and carbon dioxide and a faster rate in dissolved oxygen decline. The results indicate that these two methods of determining hypoxia tolerance in aquatic organisms are not comparable, and that much care should be given to method choice.

References

Feb 1, 1992·Journal of Applied Physiology·E D Stevens
Feb 13, 2001·Journal of Experimental Marine Biology and Ecology·S M. Porter
May 31, 2002·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·D J McKenzieJ F Steffensen
Oct 26, 2002·Marine Pollution Bulletin·D J Randall, T K N Tsui
Nov 22, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Lauren J ChapmanAmanda E Rosenberger
Apr 19, 2003·The Journal of Experimental Biology·D J McKenzieJ F Steffensen
Sep 11, 2003·The Journal of Experimental Biology·Jørund SollidGöran E Nilsson
Apr 23, 2004·Proceedings. Biological Sciences·Göran E Nilsson, Sara Ostlund-Nilsson
Aug 16, 2008·Science·Robert J Diaz, Rutger Rosenberg
Nov 11, 2008·Proceedings. Biological Sciences·Milica MandicJeffrey G Richards
Mar 18, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Göran E NilssonPhilip L Munday
May 1, 2009·Journal of Fish Biology·A R PurscheM D Taylor
Jan 11, 2013·Physiological and Biochemical Zoology : PBZ·Milica MandicJeffrey G Richards
Jul 12, 2013·The Journal of Experimental Biology·Timothy D ClarkFredrik Jutfelt
Jan 1, 1989·Fish Physiology and Biochemistry·J F Steffensen
Dec 26, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Daniel L Aboagye, Peter J Allen
Feb 19, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Erika J Eliason, Anthony P Farrell
Jan 16, 2016·Journal of Fish Biology·D ChabotA P Farrell

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Citations

Jun 14, 2016·Conservation Physiology·Nicholas J RogersRod W Wilson
May 10, 2017·The Journal of Experimental Biology·Matthew D Regan, Jeffrey G Richards
Jun 23, 2016·Journal of Fish Biology·Y K PanA J Esbaugh
Nov 14, 2018·The Journal of Experimental Biology·Chris M Wood
Oct 24, 2019·Scientific Reports·Daniel W MontgomeryRod W Wilson
Jul 28, 2017·Scientific Reports·N A HerbertA Kunzmann
Apr 4, 2019·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Tristan J McArleyNeill A Herbert
Sep 13, 2019·The Journal of Experimental Biology·Jessica E Reemeyer, Bernard B Rees
Nov 15, 2019·The Journal of Experimental Biology·Gordon R Ultsch, Matthew D Regan
Aug 14, 2019·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Warren W BurggrenJosé Fernando Méndez-Sánchez
Mar 12, 2021·The Journal of Experimental Biology·Brad A SeibelChristina J Welsh
Aug 21, 2021·The Journal of Experimental Biology·Brad A SeibelChristina J Welsh
Sep 15, 2021·The Journal of Experimental Biology·Shaun S KillenDavid J McKenzie

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