Effect of co-treatment with doxorubicin and verapamil loaded into chitosan nanoparticles on diethylnitrosamine-induced hepatocellular carcinoma in mice.

Human & Experimental Toxicology
H E Abo MansourD M Abo-Elmatty

Abstract

This study aimed to investigate the potential role of co-treatment with doxorubicin (DOX) and verapamil (VRP) nanoparticles in experimentally induced hepatocellular carcinoma in mice and to investigate the possible mechanisms behind the potential favorable effect of the co-treatment. DOX and VRP were loaded into chitosan nanoparticles (CHNPs), and cytotoxicity of loaded and unloaded drugs against HepG2 cells was evaluated. Male albino mice were divided into eight groups (n = 15): (1) normal control, (2) diethylnitrosamine, (3) CHNPs, (4) free DOX, (5) CHNPs DOX, (6) free VRP, (7) CHNPs VRP, and (8) CHNPs DOX + CHNPs VRP. Either VRP or DOX loaded into CHNPs showed stronger growth inhibition of HepG2 cells than their free forms. DOX or VRP nanoparticles displayed pronounced anticancer activity in vivo through the decline of vascular endothelial growth factor and B cell lymphoma-2 contents in liver tissues, upregulation of antioxidant enzymes, and downregulation of multidrug resistance 1. Moreover, reduced cardiotoxicity was evident from decreased level of tumor necrosis factor-α and malondialdehyde in heart tissues coupled with decreased serum activity of creatine kinase-myocardial band and lactate dehydrogenase. Co-treatment wit...Continue Reading

References

Aug 23, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·K A JanesM J Alonso
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Jan 18, 2008·Journal of Pharmaceutical Sciences·Ales Prokop, Jeffrey M Davidson
Apr 23, 2008·Archives of Otolaryngology--head & Neck Surgery·Maura CosettiStimson P Schantz
Apr 3, 2009·Molecular Pharmaceutics·Vaishali BagalkotSangyong Jon
May 20, 2009·International Journal of Pharmaceutics·Noha NafeeClaus-Michael Lehr
Jun 16, 2009·Journal of Biochemical and Molecular Toxicology·Manjari ChaudhariP V Lakshmana Rao
Feb 26, 2011·Vascular Cell·Deboshri BanerjeeShiladitya Sengupta
Jan 1, 2008·CSH Protocols·Andrew H FischerRolf Zeller
Jan 30, 2013·Chinese Journal of Cancer Research = Chung-kuo Yen Cheng Yen Chiu·Jian-Hai GuoRen-Jie Yang
Aug 27, 2013·Advanced Drug Delivery Reviews·Niravkumar R PatelVladimir P Torchilin
Feb 21, 2014·International Journal of Molecular Sciences·Yan ChenXiang-Rong Song
May 13, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Magda NasrAmani Kazem
Jun 17, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Gozde UnsoyUfuk Gunduz
Aug 8, 2014·Journal of Drug Targeting·Vimratjeet KaurAmit K Goyal
Feb 17, 2015·Journal of Advanced Research·Hossam M AbdallahAli M El-Halawany
Mar 17, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Xiaojing ZhaoXiangliang Yang
Apr 4, 2015·Laboratory Animals·R TolbaR Weiskirchen
Mar 22, 2016·Hepatic Oncology·Josiah Cox, Steven Weinman
Apr 18, 2016·Clinical and Experimental Medicine·Nahla E El-AshmawyEslam E Abd El-Fattah
May 14, 2016·International Journal of Oncology·Lu ZhaoChristiane Josephine Bruns
Jul 1, 2016·Stem Cell Investigation·Andreas Kurtz, Su-Jun Oh
Aug 19, 2015·Nanomaterials·Seung Won ShinSoong Ho Um
Apr 15, 2017·Oncotarget·Reza Bayat MokhtariHerman Yeger
May 2, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Nahla E El-AshmawyHend E Abo Mansour
Jul 12, 2017·Journal of Carcinogenesis·Yezaz Ahmed GhouriJulie H Rowe
Aug 18, 2017·Drug Development Research·Yuan SunHongzhi Qiao

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Citations

Dec 15, 2020·International Journal of Biological Macromolecules·Parisa Maleki DanaBahman Yousefi
Jul 17, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Mohamed Fawzi KabilIbrahim M El-Sherbiny

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Methods Mentioned

BETA
enzyme linked immunosorbent assay
PCR
ELISA
transmission electronic microscopy
MDA
acetylation

Software Mentioned

MATLAB
SPSS
GraphPad Prism

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