PMID: 9555932Apr 29, 1998Paper

Effect of coacervated alpha-elastin on proliferation of vascular smooth muscle and endothelial cells

Angiology
S ItoS E Wilson

Abstract

The arterial wall injury associated with arterial graft implantation causes smooth muscle cells (SMCs) in the media to migrate and proliferate in the intima at the graft-artery junction resulting in anastomotic intimal hyperplasia (AIH). An important step in developing a small-diameter prosthesis may be to stimulate endothelialization and thereby inhibit AIH. In this study, we investigated the effect of coacervated and crosslinked alpha-elastin on proliferation of SMCs and endothelial cells (ECs) in vitro. Coacervation is an important step in the conversion of proelastin to make an elastin fiber in vivo. SMCs and ECs were prepared from porcine aortic media and endothelium, respectively. SMCs and ECs (three to five passages, 4 x 10[4] cells/well) were seeded onto 12 well plates, coated and crosslinked with 0 or 10 mg/mL of coacervated alpha-elastin. After the 1st, 2nd, or 3rd day of cultivation, proliferation was assayed by scintillation counting of [3H]-thymidine incorporation. For the 4th day only, 0, 0.1, 1, 10 mg/mL concentration of coacervated alpha-elastin was coated and crosslinked. SMC proliferation (1st, 2nd day: p<0.005; 3rd, 4th day: p<0.0001) was significantly inhibited over time and dose dependently, eg, 0.1 mg/mL (...Continue Reading

References

Nov 1, 1987·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T OoyamaY Hikita
Jan 1, 1987·Differentiation; Research in Biological Diversity·U Hedin, J Thyberg
Nov 1, 1986·The Journal of Surgical Research·G R RamalanjaonaE B Silberstein
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R GrotendorstG R Martin
Aug 29, 1980·Biochemical and Biophysical Research Communications·J O CantorI Mandl
Nov 1, 1994·The Journal of Surgical Research·J L GrayH P Greisler
Apr 1, 1997·Cardiovascular Surgery : Official Journal of the International Society for Cardiovascular Surgery·S ItoS E Wilson

❮ Previous
Next ❯

Citations

May 4, 2004·Biomaterials·Kimberly A WoodhouseCatherine M Bellingham
Dec 14, 2002·Trends in Cell Biology·Benjamin S BrookeDean Y Li
Jul 3, 2003·Trends in Cardiovascular Medicine·Benjamin S BrookeDean Y Li
Jul 21, 2009·The Journal of Biological Chemistry·Daniel V BaxAnthony S Weiss
Dec 21, 2010·Tissue Engineering. Part B, Reviews·Anna WaterhouseAnthony S Weiss
Jul 13, 2012·Advanced Drug Delivery Reviews·Suzanne M MithieuxAnthony S Weiss
Jan 29, 2013·Drug Delivery and Translational Research·Balakrishnan SivaramanAnand Ramamurthi
Dec 18, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Massimo VassalliBrigida Bochicchio
May 8, 2010·Chemical Society Reviews·Jessica F AlmineAnthony S Weiss
Mar 15, 2016·Frontiers in Pharmacology·Amandine ScandoleraLaurent Duca
Jan 8, 2008·Acta Biomaterialia·M SwierczewskaP Rajagopalan
Jul 22, 2014·Biochimica Et Biophysica Acta·Junyan Xu, Guo-Ping Shi
Oct 15, 2011·Journal of Biomedical Materials Research. Part a·Lisa Nivison-Smith, Anthony S Weiss
Dec 20, 2005·Micron : the International Research and Review Journal for Microscopy·Delfo D'AlessandroFrancesco Bianchi
Jun 5, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Yunjie WangYanhang Zhang
Nov 19, 2014·BioMed Research International·Rossella BarenghiSilvia Scaglione
Jan 1, 2012·Journal of Functional Biomaterials·Steven G WiseMartin K C Ng
Aug 6, 2010·American Journal of Physiology. Cell Physiology·Boubacar MarikoGilles Faury
May 8, 2017·ACS Biomaterials Science & Engineering·David Miranda-Nieves, Elliot L Chaikof

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.