Effect of coadministration of vancomycin and BMP-2 on cocultured Staphylococcus aureus and W-20-17 mouse bone marrow stromal cells in vitro.

Antimicrobial Agents and Chemotherapy
A H NguyenY Yang

Abstract

In this study, we aimed to establish an in vitro bacterium/bone cell coculture model system and to use this model for dose dependence studies of dual administration of antibiotics and growth factors in vitro. We examined the effect of single or dual administration of the antibiotic vancomycin (VAN) at 0 to 16 μg/ml and bone morphogenetic protein-2 (BMP-2) at 0 or 100 ng/ml on both methicillin-sensitive Staphylococcus aureus and mouse bone marrow stromal cells (W-20-17) under both mono- and coculture conditions. Cell metabolic activity, Live/Dead staining, double-stranded DNA (dsDNA) amounts, and alkaline phosphatase activity were measured to assess cell viability, proliferation, and differentiation. An interleukin-6 (IL-6) enzyme-linked immunosorbent assay (ELISA) kit was used to test the bone cell inflammation response in the presence of bacteria. Our results suggest that, when delivered together in coculture, VAN and BMP-2 maintain their primary functions as an antibiotic and a growth factor, respectively. Most interestingly, this dual-delivery type of approach has shown itself to be effective at lower concentrations of VAN than those required for an approach relying strictly on the antibiotic. It may be that BMP-2 enhances c...Continue Reading

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Citations

Apr 19, 2013·Journal of Periodontology·Monika Astasov-FrauenhofferTuomas Waltimo
Sep 2, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Bryan R Orellana, David A Puleo
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Sep 9, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Devendra H DusanePaul Stoodley
Oct 19, 2019·Tissue Engineering and Regenerative Medicine·Sun Woo JungJin Ho Lee

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