Effect of coating with lung lining fluid on the ability of fibres to produce a respiratory burst in rat alveolar macrophages

Toxicology in Vitro : an International Journal Published in Association with BIBRA
D M BrownK Donaldson

Abstract

The aim of the study was to develop a simple short-term in vitro assay which would allow us to predict the pathogenicity of fibres based on data already available from in vivo studies. Fibres were used naked (uncoated) or coated with rat IgG, or rat or sheep surfactant. The fibres were used to stimulate superoxide anion release by rat alveolar macrophages. Binding of fibres to rat alveolar macrophages was assessed by optical microscopy. Fibres used in the naked state produced little or no stimulation of superoxide anion from rat alveolar macrophages. When fibres were coated with rat IgG there was a significant increase in superoxide release for all fibre types with the exception of RCF4 and Code 100/475. When fibres were coated with rat or sheep surfactant, there was suppression of the respiratory burst for all fibre types. The observed suppression was not due to a scavenging effect by the surfactant itself, because xanthine/xanthine oxidase generated superoxide was unaffected by surfactant. The suppressive effect was shown to act directly on the macrophages. Comparing naked and coated fibres for their ability to bind to macrophages, it was shown that in general more coated fibres were bound and that increased binding was assoc...Continue Reading

References

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Citations

Apr 8, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C E FisherK Donaldson
Mar 23, 2001·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·M Dörger, F Krombach
Oct 8, 2005·Particle and Fibre Toxicology·Günter OberdörsterUNKNOWN ILSI Research Foundation/Risk Science Institute Nanomaterial Toxicity Screening Working Group
May 20, 2008·Environment International·Laura CanesiGiulio Pojana
Feb 18, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ken DonaldsonAndrew Alexander
Mar 19, 2004·Environmental Technology·J ZelinskiE Mateja-Losa
Jul 26, 2005·Inhalation Toxicology·David BernsteinUNKNOWN ILSI Risk Science Institute Working Group

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