Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells

Molecular Medicine Reports
Li-Jing RanMing-Xia Shi

Abstract

The aim of the present study was to investigate the effect of co‑culture with amniotic epithelial cells (AECs) on the biological characteristics of amniotic mesenchymal stem cells (AMSCs), to compare the expression of C‑X‑C motif chemokine receptor 4 (CXCR4) in co‑cultured AMSCs and to investigate the roles of the stromal cell‑derived factor‑1 (SDF‑1)/CXCR4 axis in the homing and migration of AMSCs. AMSCs were isolated from human amniotic membranes, purified and then differentiated into osteoblasts and adipocytes in vitro, which was verified by von Kossa Staining and Oil Red O staining. Cell viability was measured by Cell Counting kit‑8 and trypan blue assays at 24, 48 and 72 h, the expression of CXCR4 was analyzed by immunofluorescence‑based flow cytometry and reverse transcription‑quantitative polymerase chain reaction, and the migration ability of AMSCs in vitro was observed by a migration assay. The results demonstrated that cell viability (at 48 and 72 h) and survival (at 24, 48 and 72 h) in the co‑culture and serum groups were higher compared with the serum‑free group. Furthermore, CXCR4 mRNA and protein expression, and migration along the SDF‑1 gradient, in the co‑culture and serum‑free groups were higher compared with t...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 24, 2004·Journal of Hematotherapy & Stem Cell Research·Baijun FangRobert Chunhua Zhao
Mar 27, 2007·Biochemical and Biophysical Research Communications·Yangxin LiYao-Hua Song
Jul 29, 2010·Journal of Cellular Biochemistry·Silvia Díaz-PradoFrancisco J Blanco
Aug 31, 2011·Pathology·Stephen Larsen, Ian D Lewis
Jun 3, 2014·BMC Cell Biology·Rebecca A PelekanosNicholas M Fisk
May 12, 2015·Journal of Immunology Research·Marta E Castro-Manrreza, Juan J Montesinos
May 18, 2016·Biochemical and Biophysical Research Communications·Honglong ZhouRuxiang Xu
May 30, 2016·Methods in Molecular Biology·Maria Ester Bernardo, Franco Locatelli
Dec 31, 2016·Cell Biology International·Chao-Ming HungMon-Juan Lee
Jan 14, 2017·International Journal of Cell Biology·Peleg RiderIdan Cohen

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Citations

Dec 4, 2021·Frontiers in Medicine·Katarzyna Skowron-KandziaPiotr Czekaj

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