Effect of colchicine induced tetraploidy on morphology, cytology, essential oil composition, gene expression and antioxidant activity of Citrus limon (L.) Osbeck.

Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology
Gunasekaran BhuvaneswariMadhusudhanan Gogulramnath

Abstract

Citrus limon (L.) Osbeck cultivated all over the world is a valuable source of aromatic essential oil. To develop tetraploids of C. limon, four different concentration of colchicine (0.025, 0.05, 0.1 and 0.2%; w/v) and three varied exposure time (12, 24 and 48 h) were employed. The ploidy level of diploids (2n = 2x = 18) and tetraploids (2n = 4x = 36) were determined by direct chromosome count and confirmed by flow cytometric analyses. Successful result with maximum tetraploidy frequency was observed in plantlets developed from seeds treated with 0.025% colchicine for 24 h. Morphological and stomatal characteristics indicated that tetraploids were taller with increased leaf and root length. On the other side, the leaves of tetraploids had fewer and larger stomata with a greater number of chloroplasts in guard cells in contrast with diploids. GC-GC/MS analyses showed cyclic monoterpene, limonene had increased significantly in tetraploids and was further confirmed by HPLC quantification. RT-PCR analyses revealed unaltered expression of monoterpene synthase, sesquiterpene synthase and flavone synthase and remarkable upregulation of genes such as limonene synthase, chalcone synthase and phenylalanine ammonia lyase in tetraploids. A...Continue Reading

References

Jul 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·O I Aruoma
Jul 6, 2000·FEBS Letters·S PlanchaisC Bergounioux
Jan 1, 2009·American Journal of Botany·Douglas E SoltisPamela S Soltis
May 9, 2012·Science China. Life Sciences·Can SongYun Liu
Jan 2, 2014·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Komal GoswamiGovind Singh
Sep 1, 2015·Recent Patents on Food, Nutrition & Agriculture·Nazik E M Mustafa
Dec 31, 2015·Planta·Mariana Cansian SattlerWellington Ronildo Clarindo

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Citations

Jan 9, 2021·Journal, Genetic Engineering & Biotechnology·Saikat Gantait, Eashan Mukherjee
Jun 3, 2021·Plants·Salvatore EspositoDomenico Carputo

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

ImageJ
SPSS

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