May 1, 1976

Effect of colicin K on a membrane-associated, energy-linked function

Journal of Bacteriology
S F Sabet

Abstract

The purpose of this work was in investigate the capability of cell extracts of Escherichia coli and E. coli treated with colicin K to catalyze the following energy-dependent reverse transhydrogenase reaction: NADP + NADH + ATP in equilibrium NADPH + NAD +ADP + Pi. Under anaerobic conditions this reaction requires the presence of a specific portion of the electron transport chain, a functional energy coupling system, including an adenosine triphosphatase, enzyme, and ATP as energy source. The ATP-linked reaction was partially inhibited in French press extracts of E. coli K-12 C600 cells that had been pretreated with colicin K but not in extracts from similarly treated cells of a colicin-tolerant mutant. Ultracentrifugation of extracts yielded particulate fractions competent in catalyzing the reaction; this reaction is substantially inhibited in fractions from colicin-treated cells. The extent of inhibition increased with increasing concentration of colicin. Supernatants also supported ATP-linked formation of NADPH, but this reaction was insensitive to the colicin effect. A comparison between the requirement of the reaction in supernatant and particulate fractions suggests that the reaction in the supernatant is different from th...Continue Reading

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Mentioned in this Paper

Antibiotic Resistance, Microbial
Alkalescens-Dispar Group
Succinate Dehydrogenase
Colicin E8
NADP
DNA-dependent ATPase
NADH, NADPH Oxidoreductases
Cell-Free System
Striadyne
Hydrolysis

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