Effect of consuming endophyte-infected fescue seed on transcript abundance in the mammary gland of lactating and dry cows, as assessed by RNA sequencing

Journal of Dairy Science
Anthony V CapucoRansom L Baldwin

Abstract

Ergot alkaloids in endophyte-infected grasses inhibit prolactin secretion and reduce milk production in lactating cows. However, we previously showed that prepartum consumption of infected seed throughout the dry period did not inhibit subsequent milk production and prior exposure to bromocriptine (ergot peptide) actually increased production in the next lactation. To identify changes in the transcriptome and molecular pathways mediating the mammary gland's response to ergot alkaloids in the diet, RNA sequencing (RNA-seq) was performed on mammary tissues obtained from 22 multiparous Holstein cows exposed to 1 of 3 treatments. Starting at 90 ± 4 d prepartum, cows were fed endophyte-free fescue seed (control; CON), endophyte-free fescue seed plus 3×/wk subcutaneous injections of bromocriptine (BROMO; 0.1 mg/kg of BW), or endophyte-infected fescue seed (INF) as 10% of the diet. Cows were dried off 60 ± 2 d prepartum. Mammary biopsies from 4 (BROMO, INF) or 5 (CON) cows/treatment at each of the 3 phases were obtained: 7 d before dry off during the initial lactation (L1), mid-dry period (D), and 10 d postpartum (L2). Although tissue from the same cow was preferentially used at 3 phases (L1, D, L2), tissue from additional cows were u...Continue Reading

References

Apr 1, 1982·The Journal of Endocrinology·B P Fitzgerald, F J Cunningham
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May 23, 2007·Journal of Dairy Science·E E ConnorA V Capuco
Jun 5, 2010·Critical Reviews in Biochemistry and Molecular Biology·Benoit ViolletFabrizio Andreelli
Feb 23, 2013·Bioinformatics·Ning LengChristina Kendziorski
Jul 1, 2012·JAK-STAT·Katherine Hughes, Christine J Watson
Dec 18, 2013·Bioinformatics·Andreas KrämerStuart Tugendreich
Oct 21, 2015·Animals : an Open Access Journal From MDPI·Heather M CrawfordGeoffrey E Dahl

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Citations

Jun 27, 2019·Toxins·Rebecca K Poole, Daniel H Poole

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