Effect of contact lenses on the protein composition in tear film: a ProteinChip study.

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
C KramannFranz H Grus

Abstract

The aim of this study was to analyze and compare the effects of rigid gas permeable and soft contact lenses on the protein composition in the tear film of contact lens wearers. Wearers of soft contact lenses (CL_S, n = 13) and rigid gas permeable contact lenses (CL_H, n = 13) were recruited for this study. Thirteen non-contact lens wearers were also included as the control. Tears were collected using Schirmer strips and frozen until use. The tears were eluted and analyzed on ProteinChips SELDI-TOF (surface-enhanced laser desorption and ionization in time of flight mass spectrometry; Bio-Rad, USA) with different chromatographic surfaces (cationic and anionic exchanger and reversed phase surface). The SELDI spectra were analyzed by multivariate statistical analysis and artificial neural networks in order to find a biomarker panel which differentiates best between the groups. In order to identify protein/peptide peaks from SELDI spectra which showed a significant difference between groups, fractionated tear samples were analyzed using MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry). For validation of biomarkers, we used an antibody microarray approach. Complex patterns of tear proteins a...Continue Reading

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Citations

Jul 25, 2013·Experimental Eye Research·Alison M McDermott
Jul 25, 2013·Experimental Eye Research·Nadine von Thun Und Hohenstein-BlaulFranz H Grus
Aug 10, 2012·Thyroid : Official Journal of the American Thyroid Association·Nina MatheisGeorge J Kahaly
Jun 27, 2012·Progress in Retinal and Eye Research·Lei Zhou, Roger W Beuerman
Dec 18, 2016·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Michael J Doughty
Aug 5, 2017·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Maureen BoostZhaoran Wang
Jun 9, 2020·Current Eye Research·Marc SchargusVerena Gerlinde Frings
Jan 25, 2017·Expert Review of Proteomics·Lei Zhou, Roger W Beuerman
Aug 5, 2020·International Ophthalmology·Marc SchargusStephanie C Joachim

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