Effect of cooperation of chaperones and gene dosage on the expression of porcine PGLYRP-1 in Pichia pastoris

Applied Microbiology and Biotechnology
Jun YangLinghua Zhang

Abstract

Mammalian peptidoglycan recognition proteins (PGLYRPs) are highly conserved pattern-recognition molecules of the innate immune system with considerable bactericidal activity, which manifest their potential values for the application to food and pharmaceutical industry. However, the effective expression of porcine PGLYRP-1 in Pichia pastoris has not been reported so far. In this study, expression in P. pastoris was explored as an efficient way to produce functional porcine PGLYRP-1. Cooperation of chaperones co-expression and gene dosage (including protein disulfide isomerase (PDI)/binding protein (BiP) and pglyrp-1) were used to enhance functional expression of antimicrobial protein in P. pastoris. Overexpression of PDI was certainly able to increase secretion level of PGLYRP-1 protein because the increase in secreted PGLYRP-1 secretion was correlated with the copy numbers of PDI in high copy pglyrp-1 clones. However, co-expression of BiP was proved to be detrimental to PGLYRP-1 secretion. In addition, we also found that excessive expression of PDI and/or BiP could decrease the mRNA expression of pglyrp-1 gene. This showed that PDI and BiP as the target genes of unfolded protein response (UPR) might regulate the transcription o...Continue Reading

References

Mar 21, 1991·Journal of Immunological Methods·R I LehrerP Eisenhauer
Jan 1, 1995·Annual Review of Immunology·H G Boman
Dec 9, 1997·Applied Microbiology and Biotechnology·M C LoewenA J Daugulis
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·D KangH Steiner
Dec 1, 2000·Molecular Biotechnology·J M CreggD R Higgins
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
Aug 22, 2003·Journal of Internal Medicine·H G Boman
Sep 19, 2003·Antioxidants & Redox Signaling·Dmitri E Fomenko, Vadim N Gladyshev
Feb 3, 2004·Biotechnology and Bioengineering·Hubertus HohenblumDiethard Mattanovich
May 26, 2004·Biochimica Et Biophysica Acta·Bonney Wilkinson, Hiram F Gilbert
Jul 13, 2004·Trends in Biotechnology·Claes GustafssonJeremy Minshull
Feb 11, 2005·Yeast·Sue Macauley-PatrickLinda M Harvey
Aug 9, 2005·Applied and Environmental Microbiology·Tiziana LodiClaudia Donnini
Oct 29, 2005·Biotechnology and Bioengineering·Mehmet InanMichael M Meagher
Dec 16, 2005·The Journal of Biological Chemistry·Xiaofeng LuRoman Dziarski
Jan 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·C Chace TydellMichael E Selsted
Jun 20, 2006·Current Opinion in Biotechnology·Dominic Esposito, Deb K Chatterjee
Aug 26, 2006·Genome Biology·Roman Dziarski, Dipika Gupta
Feb 10, 2007·The FEBS Journal·Hiderou Yoshida
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sangwoo ChoRoy A Mariuzza
Jun 10, 2008·Nucleic Acids Research·Franz S HartnerAnton Glieder
Feb 9, 2010·Applied Microbiology and Biotechnology·Alimjan IdirisKaoru Takegawa
Mar 29, 2011·BMC Evolutionary Biology·Adriana M MontañoYoko Satta
Jun 15, 2011·Veterinary Immunology and Immunopathology·Wataru UedaHaruki Kitazawa
Jan 31, 2012·Biotechnology Letters·Shuli LiangYanrui Ye
Dec 10, 2013·Journal of Theoretical Biology·Xiaowu LiuXunzhang Wang

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Citations

Mar 25, 2021·Microbial Cell Factories·Javier Garrigós-MartínezXavier Garcia-Ortega
Dec 16, 2021·Critical Reviews in Food Science and Nutrition·Zhi-Xiang XiangJin-Song Shi
Jan 9, 2022·Applied Biochemistry and Biotechnology·Subbi Rami Reddy TadiSenthilkumar Sivaprakasam

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