Effect of copper nanoparticles on physico-chemical properties of chitosan and gelatin-based scaffold developed for skin tissue engineering application

3 Biotech
Shikha KumariPradeep Srivastava

Abstract

Development of new and effective scaffold continues to be an area of intense research in skin tissue engineering. The objective of this study was to study the effect of copper nanoparticles over physico-chemical properties of the chitosan and gelatin composite scaffolds for skin tissue engineering. The copper-doped scaffolds were prepared using freeze-drying method. Chitosan and gelatin were taken in varied composition with 0.01%, 0.02%, and 0.03% Cu nanoparticles. The physico-chemical properties of the copper nanoparticles and the scaffolds were analyzed using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy. Porosity of the scaffolds was measured by liquid displacement method and hemocompatibility was tested using goat blood. SEM micrographs of the scaffolds displayed the interconnected pores which ranged between 25 and 40 µm. This average pore size was later enhanced to 95 µm after the addition of copper nanoparticles. Cell viability assay was performed to ensure the growth and proliferation of the skin cells over the scaffolds. FTIR, EDS, and XRD analysis of scaffolds confirmed the presence of copper in the chitosan-based scaffo...Continue Reading

References

Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·I V YannasG F Murphy
May 15, 1998·Journal of Cellular Biochemistry·G F Hu
Nov 24, 2001·Advanced Drug Delivery Reviews·H UenoT Fujinaga
Apr 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Chandan K SenSashwati Roy
Apr 30, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Abul Kalam AzadWillem Frans Stevens
May 16, 2008·Nature·Geoffrey C GurtnerMichael T Longaker
Aug 13, 2008·The Journal of Trauma·Rungsinee A LiusuwanDavid G Greenhalgh
Oct 27, 2009·Biomaterials·Catherine GérardCharles J Doillon
Sep 18, 2012·Trends in Biotechnology·Lara YildirimerAlexander M Seifalian
Oct 20, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·A KummrowJ Neukammer
Apr 19, 2013·Biological Trace Element Research·Ursula MirastschijskiMagnus S Ågren
Apr 23, 2013·Journal of Biomedical Materials Research. Part a·Soumi Dey SarkarSantanu Dhara
Mar 19, 2014·European Journal of Pharmacology·Anu GopalDinesh Kumar
Sep 18, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sheetal D'MelloAliasger K Salem

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Citations

May 11, 2021·International Journal of Biological Macromolecules·Farzad SeidiMasoud Mozafari
May 25, 2021·Journal of the Mechanical Behavior of Biomedical Materials·M ShirzadM Bodaghi
Jun 8, 2021·Journal of Orthopaedic Translation·Yufeng WangQingqiang Yao

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