Effect of corticosteroids on nitric oxide production in inflammatory bowel disease: are leukocytes the site of action?

American Journal of Physiology. Gastrointestinal and Liver Physiology
John D LinehanJ Westwick

Abstract

Nitric oxide (NO) production is increased in the human colonic mucosa in intestinal inflammation. We examined the effect of corticosteroids and the role of mononuclear cells in this production. Colonic biopsies from patients with ulcerative colitis and normal controls were cultured with either budesonide or prednisolone in the presence of proinflammatory cytokines. Human mixed mononuclear cells (MMCs) were cocultured with HT-29 cells stimulated with IFN-gamma and LPS in the presence or absence of corticosteroids. Nitrite production was measured in supernatants by a modification of the Griess reaction, and inducible NO synthase (iNOS) mRNA expression was studied in colonic tissue by RT-PCR. Both steroids significantly suppressed the nitrite production and iNOS mRNA expression in inflamed colonic biopsies from ulcerative colitis patients and in cytokine-stimulated normal colonic biopsies but not in cytokine-stimulated HT-29 cells. Nitrite production by HT-29 cells was significantly increased (P < 0.01) in cocultures with MMCs stimulated with IFN-gamma and LPS. The presence of either prednisolone or budesonide significantly (P < 0.01) suppressed nitrite production from cocultures of HT-29 cells and MMCs but not from cultures of HT...Continue Reading

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Citations

Nov 2, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Aline WitaicenisLuiz Claudio Di Stasi
Feb 8, 2011·Antioxidants & Redox Signaling·Lena J JohnJörg-Dieter Schulzke
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Jun 30, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·C CirilloR Cuomo
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Sep 8, 2006·European Journal of Gastroenterology & Hepatology·Ole K BonderupPeter S Teglbjaerg
Jul 14, 2020·World Journal of Gastroenterology : WJG·Aida KamalianAmir Hossein Abdolghaffari

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