Effect of Crohn's disease on bone metabolism in vitro: a role for interleukin-6

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Francisco A SylvesterGloria A Gronowicz

Abstract

Circulating proinflammatory cytokines may be involved in osteopenia associated with Crohn's disease (CD). Therefore, the effect of interleukin (IL)-6, IL-1beta, and tumor necrosis factor (TNF) a contained in Crohn's serum on bone formation was examined in a bone organ culture system. Initially, serum levels of IL-6, IL-1beta, and TNF-a were determined by ELISA in newly diagnosed, untreated children with CD and healthy age-matched controls. Serum IL-6 levels were significantly higher in patients with CD than in controls (23.9 +/- 2.8 pg/ml vs. 0.7 pg/ml +/- 0.2; p < 0.001), whereas IL-1beta and TNF-alpha serum levels were not. In the organ culture studies, 20-day-old fetal rat parietal bones were incubated for 96 h with CD or control serum, serum preincubated with a neutralizing antibody to each cytokine or a nonimmune immunoglobulin control, and with IL-6. Bone formation measured by assaying calcium content and dry weight was significantly decreased in bones exposed to Crohn's serum. Light microscopy of the bones treated with CD serum revealed a discontinuous, uneven mineralized bone matrix and disorganized osteoblasts with altered morphology. Incubation with an antibody that neutralized IL-6 activity prevented the change in os...Continue Reading

References

Jan 15, 1979·Analytical Biochemistry·C F BrunkT W James
May 1, 1991·Journal of Pediatric Gastroenterology and Nutrition·J S HyamsJ T Boyle
Feb 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A J LittlewoodM Gowen
Feb 27, 1992·The New England Journal of Medicine·J S FinkelsteinA Klibanski
Feb 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M A Fang, T J Hahn
Feb 1, 1991·Journal of Pediatric Gastroenterology and Nutrition·J S HyamsR E Moore
Jun 1, 1989·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G GronowiczL G Raisz
Jul 1, 1972·Developmental Biology·R F Diegelmann, B Peterkofsky
Jun 1, 1995·The Journal of Clinical Investigation·T BellidoS C Manolagas
Feb 2, 1995·The New England Journal of Medicine·S C Manolagas, R L Jilka
Feb 1, 1995·Gastroenterology·V AbitbolB Amor
Feb 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G A Gronowicz, M E Derome
Nov 1, 1993·Journal of Pediatric Gastroenterology and Nutrition·R M IssenmanL Fraher
Jan 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J A HoylandP T Sharpe
Apr 1, 1993·Bone and Mineral·F J Hughes, G L Howells
May 1, 1996·The Journal of Clinical Endocrinology and Metabolism·R D DevlinG D Roodman
Aug 1, 1996·Journal of Pediatric Gastroenterology and Nutrition·D A BrossA Bousvaros
Dec 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S J VargasJ A Lorenzo
May 1, 1997·Gastroenterology·E J SemeaoD A Piccoli
Mar 1, 1997·Journal of Pediatric Gastroenterology and Nutrition·J S HyamsG A Gronowicz
Oct 6, 1997·The Journal of Clinical Investigation·N FranchimontE Canalis
Mar 28, 1998·The Journal of Experimental Medicine·T AlonziG Ciliberto
May 30, 1998·Gastroenterology·R GokhaleB S Kirschner
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·S OhshimaT Kishimoto
Aug 26, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K KurokouchiH Seo
Sep 11, 1998·The American Journal of Gastroenterology·R D PollakD Rachmilewitz
Oct 27, 1998·The Journal of Pediatrics·J S Hyams, P E Hyman
Sep 24, 1999·The Journal of Clinical Investigation·S BassE Seeman
Oct 6, 2000·The Journal of Clinical Investigation·J DaiE T Keller
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·F De BenedettiA Martini

❮ Previous
Next ❯

Citations

Oct 18, 2013·European Journal of Pediatrics·Sze Choong WongMargaret Zacharin
Apr 25, 2014·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·A TsampalierosM B Leonard
Sep 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Andrew SawczenkoIan R Sanderson
Oct 5, 2013·Journal of Crohn's & Colitis·Piero VerniaSanti Cannizzaro
Jan 1, 2008·Reviews in Endocrine & Metabolic Disorders·Anuradha Viswanathan, Francisco A Sylvester
Oct 1, 2008·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·A KuwabaraS Kido
Jun 9, 2009·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·L M WardD R Mack
Jun 11, 2004·Annual Review of Nutrition·S A Abrams, K O O'Brien
Mar 11, 2008·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·D DelalandeI Koné-Paut
Jul 29, 2009·Inflammatory Bowel Diseases·Susanne BechtoldSibylle Koletzko
Jan 9, 2007·Inflammatory Bowel Diseases·Massimiliano PaganelliSalvatore Cucchiara
Aug 19, 2006·Inflammatory Bowel Diseases·Gary R LichtensteinMichael Pazianas
Apr 13, 2004·Journal of Pediatric Gastroenterology and Nutrition·Rob Heuschkel
May 16, 2018·Experimental Biology and Medicine·Xingtao GeYu Yang
Mar 16, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Isabelle AllaeysPatrice E Poubelle
Jan 13, 2011·The American Journal of Gastroenterology·Katharina J WerkstetterSibylle Koletzko
Jun 12, 2010·American Journal of Rhinology & Allergy·Karthik RajasekaranPete S Batra
Jul 23, 2004·Journal of Pediatric Gastroenterology and Nutrition·Stefano Mora, Graziano Barera
Jun 23, 2011·Journal of Pediatric Gastroenterology and Nutrition·Helen PappaCatherine Gordon
Aug 1, 2006·Journal of Pediatric Gastroenterology and Nutrition·S C WongS F Ahmed
Nov 30, 2006·Journal of Pediatric Gastroenterology and Nutrition·Francisco A Sylvester
Mar 8, 2005·Journal of Pediatric Gastroenterology and Nutrition·Manisha HarpavatDavid J Keljo
Apr 15, 2010·The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi·Jae Jung ParkYoung Sun Hong
Mar 25, 2005·Journal of Internal Medicine·P VestergaardL Mosekilde
Aug 21, 2007·Clinical Journal of the American Society of Nephrology : CJASN·Stuart L GoldsteinDouglas M Silverstein
Sep 1, 2010·Expert Review of Endocrinology & Metabolism·Salma Malik, S Faisal Ahmed
Nov 1, 2016·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Corinne E MetzgerSusan A Bloomfield
Nov 28, 2002·Journal of Pediatric Gastroenterology and Nutrition·Samuel VargheseFrancisco A Sylvester
Dec 12, 2019·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·A A van Bodegraven, N Bravenboer
Apr 28, 2011·Alimentary Pharmacology & Therapeutics·S G VeerappanB M Ryan
Aug 11, 2016·European Journal of Gastroenterology & Hepatology·Sundaram G VeerappanBarbara M Ryan
Mar 28, 2018·Experimental and Therapeutic Medicine·Hongyun WeiJie Zhang
Mar 4, 2015·Digestive Diseases and Sciences·Sundaram G VeerappanBarbara M Ryan
Aug 5, 2004·Inflammatory Bowel Diseases·Jesse S SiffledeenGordon Greenberg
Mar 21, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Laura HarrisLaura R McCabe
Sep 9, 2004·Alimentary Pharmacology & Therapeutics·N FranchimontE Louis
Dec 19, 2019·International Journal of Molecular Sciences·Ke KeYousef Abu-Amer
Feb 26, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip AlexXuhang Li
Dec 18, 2020·Current Opinion in Gastroenterology·Nathalie BravenboerAdriaan A van Bodegraven

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA
Yvette M HenryRichard Eastell
© 2022 Meta ULC. All rights reserved